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Current Microbiology

, Volume 41, Issue 2, pp 84–88 | Cite as

Phylogeny of Marine Bacillus Isolates from the Gulf of Mexico

  • Janet L. Siefert
  • Maia Larios-Sanz
  • Lawrence K. Nakamura
  • Ralph A. Slepecky
  • John H. Paul
  • Edward R. B. Moore
  • George E. Fox
  • Peter Jurtshuk, Jr.

Abstract

The phylogeny of 11 pigmented, aerobic, spore-forming isolates from marine sources was studied. Forty-two biochemical characteristics were examined, and a 16S rDNA sequence was obtained for each isolate. In a phylogenetic tree based on 16S sequencing, four isolates (NRRL B-14850, NRRL B-14904, NRRL B-14907, and NRRL B-14908) clustered with B. subtilis and related organisms; NRRL B-14907 was closely related to B. amyloliquefaciens. NRRL B-14907 and NRRL B-14908 were phenotypically similar to B. amyloliquefaciens and B. pumilus, respectively. Three strains (NRRL B-14906, NRRL B-14910, and NRRL B-14911) clustered in a clade that included B. firmus, B. lentus, and B. megaterium. NRRL B-14910 was closely related phenotypically and phylogenetically to B. megaterium. NRRL B-14905 clustered with the mesophilic round spore-producing species, B. fusiformis and B. sphaericus; the isolate was more closely related to B. fusiformis. NRRL B-14905 displayed characteristics typical of the B. sphaericus-like organisms. NRRL B-14909 and NRRL B-14912 clustered with the Paenibacillus species and displayed characteristics typical of the genus. Only NRRL B-14851, an unusually thin rod that forms very small spores, may represent a new Bacillus species.

Keywords

Bacillus Phylogenetic Tree rDNA Sequence Biochemical Characteristic Bacillus Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc. 2000

Authors and Affiliations

  • Janet L. Siefert
    • 1
  • Maia Larios-Sanz
    • 1
  • Lawrence K. Nakamura
    • 2
  • Ralph A. Slepecky
    • 3
  • John H. Paul
    • 4
  • Edward R. B. Moore
    • 5
  • George E. Fox
    • 1
  • Peter Jurtshuk, Jr.
    • 1
  1. 1.Dept. of Biology and Biochemistry, University of Houston, Houston, TX 77204-5513, USAUS
  2. 2.National Center for Agricultural Utilization Research, U.S. Dept. of Agriculture, 1815 N. University St., Peoria, IL 61604, USAUS
  3. 3.Dept. of Biology, Syracuse University, Syracuse, NY 13244, USAUS
  4. 4.Dept. of Marine Science, Univ. of South Florida, St. Petersburg, FL 33701, USAUS
  5. 5.Division of Microbiology, GBF-National Research Institute for Biotechnology, Mascheroder Weg 1, Braunschweig D-38124, GermanyDE

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