Advertisement

Current Microbiology

, Volume 40, Issue 6, pp 362–366 | Cite as

Isolation of a Gene from Burkholderia cepacia IS-16 Encoding a Protein That Facilitates Phosphatase Activity

  • Hilda  Rodríguez
  • Gian M.  Rossolini
  • Tania  Gonzalez
  • Jiping  Li
  • Bernard R.  Glick

Abstract.

A genomic library from Burkholderia cepacia IS-16 was constructed in Escherichia coli by partial Sau3AI digestion of the chromosomal DNA, with the plasmid vector Bluescript SK. This library was screened for clones able to grow as green stained colonies on selective medium developed for detecting phosphatase-positive colonies. Three green-stained clones (pFS1, pFS2, and pFS3) carried recombinant plasmids harboring DNA inserts of 5.0, 8.0, and 0.9 kb, respectively. DNA hybridization experiments demonstrated the presence of overlapping DNA fragments in the three clones and that these three clones were all derived from Burkholderia cepacia IS-16 genomic DNA. DNA sequence analysis, together with polyacrylamide gels of proteins encoded by E. coli containing pFS3, suggested that the isolated 0.9-kb DNA fragment encodes the functional portion of a phosphate transport protein.

Keywords

Phosphatase Activity Recombinant Plasmid Selective Medium Plasmid Vector Genomic Library 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag New York Inc. 2000

Authors and Affiliations

  • Hilda  Rodríguez
    • 1
  • Gian M.  Rossolini
    • 2
  • Tania  Gonzalez
    • 1
  • Jiping  Li
    • 3
  • Bernard R.  Glick
    • 3
  1. 1.Department of Microbiology, Cuban Research Institute on Sugarcane By-Products, Havana, Cuba CU
  2. 2.Department of Molecular Biology, University of Siena, Italy IT
  3. 3.Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1 CA

Personalised recommendations