Optimizing the Reduction of Molybdate by Two Novel Thermophilic Bacilli Isolated from Sinai, Egypt

  • Ali M. SaeedEmail author
  • Hayam A. E. Sayed
  • Einas H. El-Shatoury


The aim of this study was to isolate thermophilic bacteria to be used in in situ bioremediation of molybdate at elevated temperatures. Two molybdate reducing bacteria (Pharon2 and Pharon3) were isolated from the hot Spring at Pharaoh's Bath, Sinai, Egypt. The isolates were identified by 16S rRNA genes sequencing and were submitted to GenBank as Bacillus tequilensis strain Pharon2 (MK078034) and Bacillus sonorensis strain Pharon3 (MK078035). The molybdenum blue production was optimized using multifactorial statistical approaches, Plackett–Burman and central composite designs. According to the results obtained by response optimizer, the maximum molybdenum blue production achieved was 1.04 and 1.12 represented as absorbance at 865 nm, with the optimum salt concentration of 1.1 and 2.5%; at pH 7.02 and 7.07; incubation temperature of 46.1 and 52.2 °C; sucrose and glucose as an electron donor for Bacillus tequilensis strain Pharon2 and Bacillus sonorensis strain Pharon3, respectively. In conclusion, the thermophilic bacterial isolates belonging to the genus Bacillus could be used in in situ bioremediation under elevated temperatures. To the best of our knowledge, this is the first record of molybdenum blue production by thermophilic Bacillus tequilensis and Bacillus sonorensis.



The authors are greatly thankful to the Department of Microbiology, Faculty of Science, Ain Shams. University, Egypt and to Ms. Amira Gamal, Assistant lecturer at Egyptian Atomic Energy Authority for her collaboration in Statistical factorial designs.

Compliance with Ethical Standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Research Involving Human and Animal Rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

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Supplementary file1 (DOCX 39 kb)


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© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Microbiology, Faculty of ScienceAin Shams UniversityCairoEgypt

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