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Chryseotalea sanaruensis gen. nov., sp., nov., a Member of the Family Cytophagaceae, Isolated from a Brackish Lake in Hamamatsu Japan

  • Yoshiaki Maejima
  • Takao Iino
  • Yusuke Muraguchi
  • Kohei Fukuda
  • Moriya Ohkuma
  • Tomohiro Suzuki
  • Ryota Moriuchi
  • Hideo Dohra
  • Kazuhide Kimbara
  • Masaki ShintaniEmail author
Article

Abstract

A strain-designated YsT was isolated as a filterable bacterial strain from Lake Sanaru, a brackish water lake in Hamamatsu Japan. YsT is aerobic, Gram-negative, and slender rod shaped. YsT grew optimally at 30 °C, pH 7.0–8.0 and without the addition of NaCl. MK-7 was the sole isoprenoid quinone. The main cellular polar lipids were phosphatidylethanolamine and unidentified amino- and polar-lipids. The predominant cellular fatty acids were C18:0, iso-C14:0 and iso-C15:0. Phylogenetic analysis of 16S rRNA gene sequence revealed the nearest neighbours of strain YsT to be members of the Ohtaekwangia and Chryseolinea genera with 91.2–92.1% sequence similarity. The percentages of conserved proteins (POCP) between the genomes of YsT and related strains were less than 50%. Phenotypic analyses suggested that YsT could not metabolize glucose and related sugars, which was discriminative from its phylogenetic relatives. We, therefore, propose a novel species in a new genus, Chryseotalea sanaruensis gen. nov., sp. nov. in the family Cytophagaceae (= JCM 30318T = LMG 30359T), based on cell size, the predominant cellular fatty acid composition, and the DNA GC content (38.9 mol%).

Notes

Funding

This study was supported by the Institute for Fermentation, Osaka (IFO), Japan.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Human and Animal Rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

284_2019_1823_MOESM1_ESM.pdf (2.3 mb)
Supplementary file1 (PDF 2326 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Yoshiaki Maejima
    • 1
  • Takao Iino
    • 2
  • Yusuke Muraguchi
    • 1
  • Kohei Fukuda
    • 3
  • Moriya Ohkuma
    • 2
  • Tomohiro Suzuki
    • 4
    • 5
  • Ryota Moriuchi
    • 4
  • Hideo Dohra
    • 4
  • Kazuhide Kimbara
    • 1
  • Masaki Shintani
    • 1
    • 2
    • 3
    • 4
    Email author
  1. 1.Department of Engineering, Graduate School of Integrated Science and TechnologyShizuoka UniversityShizuokaJapan
  2. 2.Japan Collection of Microorganisms RIKEN, BioResource Research CenterTsukubaJapan
  3. 3.Department of Bioscience, Graduate School of Science and TechnologyShizuoka UniversityShizuokaJapan
  4. 4.Research Institute of Green Science and TechnologyShizuoka UniversityShizuokaJapan
  5. 5.Center for Bioscience Research and EducationUtsunomiya UniversityUtsunomiyaJapan

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