Chryseotalea sanaruensis gen. nov., sp., nov., a Member of the Family Cytophagaceae, Isolated from a Brackish Lake in Hamamatsu Japan
A strain-designated YsT was isolated as a filterable bacterial strain from Lake Sanaru, a brackish water lake in Hamamatsu Japan. YsT is aerobic, Gram-negative, and slender rod shaped. YsT grew optimally at 30 °C, pH 7.0–8.0 and without the addition of NaCl. MK-7 was the sole isoprenoid quinone. The main cellular polar lipids were phosphatidylethanolamine and unidentified amino- and polar-lipids. The predominant cellular fatty acids were C18:0, iso-C14:0 and iso-C15:0. Phylogenetic analysis of 16S rRNA gene sequence revealed the nearest neighbours of strain YsT to be members of the Ohtaekwangia and Chryseolinea genera with 91.2–92.1% sequence similarity. The percentages of conserved proteins (POCP) between the genomes of YsT and related strains were less than 50%. Phenotypic analyses suggested that YsT could not metabolize glucose and related sugars, which was discriminative from its phylogenetic relatives. We, therefore, propose a novel species in a new genus, Chryseotalea sanaruensis gen. nov., sp. nov. in the family Cytophagaceae (= JCM 30318T = LMG 30359T), based on cell size, the predominant cellular fatty acid composition, and the DNA GC content (38.9 mol%).
This study was supported by the Institute for Fermentation, Osaka (IFO), Japan.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
Human and Animal Rights
This article does not contain any studies with human participants or animals performed by any of the authors.
- 7.Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acids techniques in bacterial systematics. Wiley, Chichester, pp 115–147Google Scholar
- 9.Maejima Y, Kushimoto K, Muraguchi Y, Fukuda K, Miura T, Yamazoe A, Kimbara K, Shintani M (2018) Proteobacteria and Bacteroidetes are major phyla of filterable bacteria passing through 0.22 mum pore size membrane filter, in Lake Sanaru, Hamamatsu, Japan. Biosci Biotechnol Biochem 82(7):1260–1263Google Scholar
- 12.Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note101. MIDI Inc, Newark, DEGoogle Scholar
- 17.Weimann A, Mooren K, Frank J, Pope PB, Bremges A, McHardy AC (2016) From genomes to phenotypes: traitar, the microbial trait analyzer. mSystems 1(6): e00101-16. https://doi.org/10.1128/mSystems.00101-16