Antibacterial Effects of Schisandra chinensis Extract on Escherichia coli and its Applications in Cosmetic
- 63 Downloads
Schisandra chinensis (Turcz.) Baill (S. chinensis), an edible traditional medicine herb, has a strong constitution, which extract has good antibacterial activity. The study investigated its antibacterial properties on E. coli, to find a candidate for the development as new preservative. In vitro antibacterial assay showed that S. chinensis extract (SCE) effectively inhibited the growth of test bacteria with MBC of 18 mg/mL. In model cosmetic system of O/W emulsions, SCE possessed a great antibacterial capacity. The growth curves of E. coli treated with SCE exhibited an extended lag phase and restricted log phase. Scanning electron microscopy showed that the treated E. coli cells exhibited wrinkled and withered surfaces, and disappearing outmost layer, suggesting S. chinensis extract can damage S. aureus cell member and wall, in addition, the leakage of periplasm enzyme AKPase and the increased activities of Na+/K+-ATPase and Ca++-ATPase in cell membrane were also consistent with the microscopy results. Moreover, the S. chinensis extract can decrease the activities of dehydrogenase and total ATPase and the content of intracellular proteins, and bind with S. aureus DNA by electrostatic and groove binding. The results indicated that SCE might be a candidate cosmetic preservative for its good antibacterial activity and multiple targets on E. coli.
This work was financially supported by the National Natural Science Foundation of China (Grant No. 31401390).
- 1.Szopa A, Ekiert R, Ekiert H (2017) Current knowledge of Schisandra chinensis (Turcz.) Baill. (Chinese magnolia vine) as a medicinal plant species: a review on the bioactive components, pharmacological properties, analytical and biotechnological studies. Phytochem Rev 16(2):195–218CrossRefGoogle Scholar
- 3.Kang YS, Han MH, Hong SH, Park C, Hwang HJ, Kim BW, Kyoung KH, Choi YW, Kim CM, Choi YH (2014) Anti-inflammatory effects of Schisandra chinensis (Turcz.) Baill fruit through the inactivation of nuclear factor-κB and mitogen-activated protein kinases signaling pathways in lipopolysaccharide-stimulated murine macrophages. J Cancer Prev 19(4):279–287CrossRefGoogle Scholar
- 4.Yue C, Chen J, Hou R, Liu J, Li X, Gao Z, Liu C, Wang D, Yu L, Li H (2015) Effects of selenylation modification on antioxidative activities of Schisandra chinensis polysaccharide. PLoS ONE 10(7):e134363Google Scholar
- 6.Xu L, Grandi N, Del VC, Mandas D, Corona A, Piano D, Esposito F, Parolin C, Tramontano E (2015) From the traditional Chinese medicine plant Schisandra chinensis new scaffolds effective on HIV-1 reverse transcriptase resistant to non-nucleoside inhibitors. J Microbiol 53(4):288–293CrossRefGoogle Scholar
- 16.Opletal L, Krenková M, Havlícková P (2001) Phytotherapeutic aspects of diseases of the circulatory system 8 Chinese magnolia (Schisandra chinensis (Turcz.) Baill.): production of the drugs and their evaluation, therapeutic and dietary preparations. Ceská a Slovenská farmacie: casopis Ceské farmaceutické spolecnosti a Slovenské farmaceutické spolecnosti 50(5):219–224Google Scholar
- 17.Ryool, KY, Hwan, CI, Seog, SG, Seok, MB, Hye, PC, Mee, CY, Eok, KO, and Kyung, CY (2011). Composition useful as health functional food composition for preventing, improving and treating obesity, preferably growing obesity due to increased number of adipocytes, comprises mixed extract of Aronia and Schisandra chinensis.Google Scholar
- 18.Hyun, CY, Woo, JJ, Choieunok, Lee, KW, Ki, YK, Sung, GK, Kim, S, Hong, SH, and P, C (2018). Composition useful as quasi drug composition and food composition for preventing and treating arthritis including osteoarthritis and rheumatoid arthritis, comprises Schisandra chinensis extracted by freezing Schisandra, p. 1294732238.Google Scholar
- 20.Huang Q, Zhou M, Ye Z (2001) Effect of amicarthiazol to the cell of Xanthomonas citri. J Nanjing Agric Univ 24(3):23–26Google Scholar
- 21.Fan L, Tong Q, Dong W, Yang G, Hou X, Xiong W, Shi C, Fang J, Wang WJOF (2014) Tissue distribution, excretion, and metabolic profile of dihydromyricetin, a flavonoid from vine tea (Ampelopsis grossedentata) after oral administration in rats. J Funct Foods 9(1):290–294Google Scholar