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Lysobacter segetis sp. nov., Isolated from Soil

  • Leonid N. Ten
  • Jongguen Jeon
  • Nabil Salah Elderiny
  • Myung Kyum Kim
  • Seung-Yeol Lee
  • Hee-Young JungEmail author
Article
  • 47 Downloads

Abstract

A Gram-negative, aerobic, motile by gliding, rod-shaped bacterium, strain 17J68-2T, was isolated from a soil sample taken from Jeju Island, Republic of Korea. The isolate displayed high 16S rRNA gene sequence similarity to the members of the genus Lysobacter in the family Lysobacteraceae, with Lysobacter humi FJY8T (98.4% similarity), Lysobacter xinjiangensis RCML-52T (98.3%), and Lysobacter mobilis 9NM-14T (98.1%) as closest phylogenetic neighbors. Growth of strain 17J68-2T occurred at 15–42 °C, pH 7–8, and in the presence of 0–1.0% NaCl. Draft genome was 2.94 Mb in size with G+C content of 70.5 mol%. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylethanolamine. Ubiquinone Q-8 was the predominant respiratory quinone and the major fatty acids were C16:0 iso (39.4%), summed feature 3 (C16:1ω7c/C16:1ω6c) (6.6%), C11:0 iso 3–OH (6.4%), C15:0 iso (6.4%), and C16:1 iso H (6.2%). The DNA–DNA relatedness between strain 17J68-2T and L. humi, L. xinjiangensis, and L. mobilis were 39.9, 39.4, and 25.3%, respectively. From these results, it is concluded that the novel isolate possesses sufficient characteristics to differentiate it from the most closely affiliated Lysobacter species, and strain 17J68-2T represents a novel species of the genus Lysobacter, for which the name Lysobacter segetis sp. nov. (=KCTC 62237T = JCM 33058T) is proposed.

Notes

Acknowledgements

This work was supported by the Brain Pool Program (Grant No. 2019H1D3A2A01061406) through the National Research Foundation (NRF) funded by the Ministry of Science and ICT, Republic of Korea.

Supplementary material

284_2019_1801_MOESM1_ESM.pdf (448 kb)
Supplementary file1 (PDF 447 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Leonid N. Ten
    • 1
  • Jongguen Jeon
    • 1
  • Nabil Salah Elderiny
    • 1
  • Myung Kyum Kim
    • 2
  • Seung-Yeol Lee
    • 1
    • 3
  • Hee-Young Jung
    • 1
    • 3
    Email author
  1. 1.School of Applied BiosciencesKyungpook National UniversityDaeguRepublic of Korea
  2. 2.Department of Bio and Environmental TechnologySeoul Women’s UniversitySeoulRepublic of Korea
  3. 3.Institute of Plant MedicineKyungpook National UniversityDaeguRepublic of Korea

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