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Pseudomonas jilinensis sp. nov., Isolated from Oil Production Water of Jilin Oilfield in China

  • Jian-Wei Wang
  • Man Cai
  • Yong NieEmail author
  • Bing Hu
  • Ying YangEmail author
  • Xiao-Lei Wu
Review Article

Abstract

A Gram-stain-negative, rod-shaped bacterial strain JS15-10A1T was isolated from oil production water. Its optimum growth was observed at 37 °C, pH 7.0, and 3% (w/v) NaCl. The 16S rRNA gene sequence of strain JS15-10A1T showed the highest similarities with Pseudomonas parafulva CB-1T (97.6%) and P. fulva IAM 1529T (97.5%). In addition, phylogenetic analyses based on multilocus sequence analyses with concatenating 16S rRNA, gyrB, rpoD, and rpoB genes indicated that strain JS15-10A1T was a member of genus Pseudomonas but discriminated from other species. Furthermore, whole-genome analyses revealed that average nucleotide identities and in silico DNA-DNA hybridization values of strain JS15-10A1T against its closest relatives were all below 76.7% and 21.1%, respectively. The major cellular fatty acids of strain JS15-10A1T were summed feature 8 (C18:1ω7c/C18:1ω6c), C16:0, C12:0, C17:0 cyclo, summed feature 3 (C16:1ω7c/C16:1ω6c), C12:0 3-OH, C10:0 3-OH, and C19:0 cyclo ω8c. The predominant quinone was ubiquinone Q-9. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unknown amino-lipid, and two unidentified lipids. The genome DNA G + C content was 60.0 mol%. On the basis of phylogenetic, phenotypic, physiological, and chemotaxonomic analyses, it can be concluded that strain JS15-10A1T represents a novel species in genus Pseudomonas, for which the name Pseudomonas jilinensis sp. nov. is proposed. The type strain is JS15-10A1T (= CGMCC 1.16072T = LMG 30036T).

Abbreviations

PE

Phosphatidylethanolamine

PG

Phosphatidylglycerol

DPG

Diphosphatidylglycerol

AL

Unknown amino-polar lipid

L

Unknown polar lipid

ANI

Average nucleotide identity

MLSA

Multilocus sequence analyses

DDH

DNA-DNA hybridization

GGDC

Genome-to-genome distance calculator

Notes

Acknowledgements

This work was supported by the Educational Commission of Anhui Province of China (Grant Nos. 2015zytz041, 2018jyxm1444), PetroChina Science and Technology Innovation Grant (Grant No. 2016D-5007–0701), the Independent Project Program of State Key Laboratory of Petroleum Pollution Control (Gene-guiding Isolation and Screening of n-alkane Degrading Bacteria; Grant No. PPCIP2017001), and CNPC Research Institute of Safety and Environmental Technology. The authors thank the Institute of Microbiology, Chinese Academy of Sciences, for providing the transmission electron microscopy facility.

Compliance with Ethical Standards

Conflicts of interest

The authors declare that there are no conflicts of interest.

Supplementary material

284_2019_1798_MOESM1_ESM.docx (2.8 mb)
Electronic supplementary material 1 (DOCX 2823 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Anhui Province of Water Pollution Control and Wastewater ReuseAnhui Jianzhu UniversityHefeiPeople’s Republic of China
  2. 2.College of EngineeringPeking UniversityBeijingPeople’s Republic of China
  3. 3.Institute of MicrobiologyChina General Microbiological Culture Collection Center, Chinese Academy of SciencesBeijingPeople’s Republic of China
  4. 4.Department of Biochemical Engineering, College of Chemistry and Chemical EngineeringInstitute for Synthetic Biosystem, Beijing Institute of TechnologyBeijingPeople’s Republic of China

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