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Clonal Dissemination of Clinical Isolates of Acinetobacter baumannii Carriers of 16S rRNA Methylase Genes in an Oncological Hospital in Recife, Brazil

  • Jussyêgles Niedja da Paz PereiraEmail author
  • Carlos Alberto das Neves de Andrade
  • Jailton Lobo da Costa Lima
  • Reginaldo Gonçalves de Lima Neto
  • Paulo Sérgio Ramos de Araújo
  • Maria Amélia Vieira Maciel
Article

Abstract

16S rRNA methylases confer high-level resistance to aminoglycosides which are used to treat serious infections caused by gram-negative bacteria, such as Acinetobacter spp. Some genes encoding these enzymes are disseminated worldwide, while others were detected in only some countries. The objective was to characterize the susceptibility profile to aminoglycosides (amikacin and gentamicin) of clinical isolates of Acinetobacter spp. from an oncological hospital in Recife, and given the resistance to both antimicrobials, to characterize minimal inhibitory concentrations (MICs) of amikacin, gentamicin and tobramycin, the occurrence of 16S rRNA methylase genes (armA, rmtB, rmtC and rmtD) and of ß-lactamase gene (blaKPC) and the clonal profile. Isolates resistant to both antimicrobials, amikacin and gentamicin, were selected by disk diffusion technique in Mueller–Hinton agar and identified. Broth microdilution was conducted to determine MICs of amikacin, gentamicin, and tobramycin. These isolates were subjected to polymerase chain reaction and pulsed-field gel electrophoresis. Among 23 analyzed isolates, 12 (52.2%) were resistant to gentamicin and amikacin and identified as Acinetobacter baumannii. Among these, 11 (91.7%), 12 (100%), and 9 (75%) isolates showed respectively MICs > 256 µg/mL of amikacin, > 64 µg/mL of gentamicin, and > 64 µg/mL of tobramycin. The armA gene was found in 12 (100%) isolates and 6 (50%) showed coexistence of armA, rmtB, and rmtC genes. The rmtD and blaKPC genes were not detected. These isolates showed high genetic similarity (92%) and were classified as clone A. Elaboration and fulfillment of measures are thus essential to prevent the spread of this resistance mechanism.

Notes

Acknowledgements

The authors are grateful to Danilo Elias Xavier, PhD, from the Institute Aggeu Magalhães, Fiocruz, Pernambuco, Brazil, for providing the positive controls used for the execution of the PCRs of 16S rRNA methylase genes. The authors are also grateful to the Brazilian National Council for Scientific and Technological Development (CNPq) for the financial support.

Compliance with Ethical Standards

Conflicts of interest

The authors declare that there are no conflicts of interest.

Ethical Approval

This study was approved by the Human Research Ethics Committee of Pernambuco Cancer Hospital (Certificate of Presentation for Ethical Appreciation—CAAE: 05554812.7000.5205).

Supplementary material

284_2019_1786_MOESM1_ESM.pdf (604 kb)
Electronic supplementary material 1 (PDF 605 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Jussyêgles Niedja da Paz Pereira
    • 1
    Email author
  • Carlos Alberto das Neves de Andrade
    • 2
  • Jailton Lobo da Costa Lima
    • 1
  • Reginaldo Gonçalves de Lima Neto
    • 1
  • Paulo Sérgio Ramos de Araújo
    • 1
    • 2
  • Maria Amélia Vieira Maciel
    • 1
  1. 1.Departamento de Medicina Tropical/Universidade Federal de PernambucoRecifeBrazil
  2. 2.Instituto Aggeu Magalhães RecifeBrazil

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