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Current Microbiology

, Volume 76, Issue 12, pp 1495–1502 | Cite as

Comparison of Starvation-Induced Persister Cells with Antibiotic-Induced Persister Cells

  • Shridhar S. Paranjape
  • Ravindranath ShashidharEmail author
Article

Abstract

The phenotypic heterogeneity in a large population arises because of fluctuation in microenvironments and stochastic gene expressions. In this report, we isolated two types of persistent sub-populations of Vibrio cholerae, one triggered by starvation and another by antibiotics. We characterised starvation-induced (E-cells) and antibiotic-induced (P-cell) persister cells for stress tolerance, colony morphology and toxin gene expressions. Both the sub-populations differ with respect to morphology, temperature tolerance and oxidative stress tolerance. The E-cells were smaller than the P-cells and formed tiny colonies (1–2 mm). The E-cells were more sensitive to heat and oxidative stress compared with P-cells. The up-regulated genes of P-cells include, genes of antioxidant enzymes (>5 fold), cholera toxin (>26 fold) and toxin: antitoxin protein hipA (>100 fold). Upon nutrient up-shift, the E-cells recovered after lag time of 6 h. However, such lag extension was not visible during P-cell recovery, suggesting that P-cell physiology is more akin to normal cells than E-cells. This is the first comparative report on the two different persister sub-populations of V. cholerae. The E-cells and P-cells are similar regarding antibiotic tolerance. However, the sub-populations differ significantly in stress tolerance and other phenotypes studied.

Notes

Funding

Funding was provided by Department of Atomic Energy, India.

Supplementary material

284_2019_1777_MOESM1_ESM.docx (131 kb)
Supplementary file1 (DOCX 131 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Shridhar S. Paranjape
    • 1
    • 2
  • Ravindranath Shashidhar
    • 1
    • 2
    Email author
  1. 1.Food Technology DivisionBhabha Atomic Research CentreMumbaiIndia
  2. 2.Life SciencesHomi Bhabha National Institute (Deemed To Be University)MumbaiIndia

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