Prospecting Plant Growth-Promoting Bacteria Isolated from the Rhizosphere of Sugarcane Under Drought Stress
In the rhizosphere, the soil bacteria and the plants are closely related, with the plant-associated microbiota playing an important role in promoting plant growth under both normal and stress conditions. In this study, the cultivable bacteria in the sugarcane rhizosphere under different levels of drought stress were characterized and screened for plant growth activities. The results suggested that the microbial community associated with the sugarcane rhizosphere was strongly affected by drought, but some important genera of bacteria such as Arthrobacter, Pseudomonas, Microbacterium, and Bacillus remained present during the entire experiment, indicating the adaptability of these organisms and their importance in the rhizosphere community. Many isolates exhibited positive results for one or more plant growth activity, and they were also capable of growing under simulated drought stress, suggesting that the microorganisms isolated from the sugarcane rhizosphere could be explored for uses such as biofertilizers or biocontrol agents in agriculture.
This work was supported by the São Paulo State Research Foundation (FAPESP, Grant Number 2015/00408-5). L.B.P. received a fellowship from FAPESP/CAPES (Grant Number 2014/05929-0) and National Council for Scientific and Technological Development (CNPq, Grant Number 140547/2014-2).
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 3.Someya N, Akutsu K (2005) Biocontrol of plant diseases by genetically modified microorganisms: current status and future prospects. In: Siddiqui ZA (ed) PGPR: biocontrol and biofertilization. Springer, Dordrecht, pp 297–312Google Scholar
- 14.Cane Technology Center—CTC (2012) Varieties CTC: varieties highlights. CTC, PiracicabaGoogle Scholar
- 15.Sambrook E, Fritsch TM, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
- 26.Hammer Ø, Harper DAT, Ryan PD (2001) PAST: paleontological statistics software package for education and data analysis. Palaeontol Electron 4(1):1–9Google Scholar
- 28.Griffiths RI, Whiteley AS, Anthony G, Donnell O (2003) Physiological and community responses of established grassland bacterial populations to water stress physiological and community responses of established grassland bacterial populations to water stress. Appl Environ Microbiol 69(12):6961–6968CrossRefPubMedPubMedCentralGoogle Scholar
- 31.Dhanraj BN (2013) Bacterial diversity in sugarcane (Saccharum officinarum) rhizosphere of saline soil. Int Res J Biol Sci 2:60–64Google Scholar
- 41.Akhtar N, Ali A, Bashir U, Haider MS (2011) Morphological and biochemical studies on bacterial microfauna from lahore soils. Pak J Phytopathol 25:137–140Google Scholar