Complete Genome Sequence of Paenibacillus sp. CAA11: A Promising Microbial Host for Lignocellulosic Biorefinery with Consolidated Processing

  • Gyeongtaek Gong
  • Hyun Ju Oh
  • Sukhyeong Cho
  • Seil Kim
  • Min-Kyu Oh
  • Youngsoon Um
  • Sun-Mi LeeEmail author


Several bioprocessing technologies, such as separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and consolidated bioprocessing (CBP), have been highlighted to produce bio-based fuels and chemicals from lignocellulosic biomass. Successful CBP, an efficient and economical lignocellulosic biorefinery process compared with other processes, requires microorganisms with sufficient cellulolytic activity and biofuel/chemical-producing ability. Here, we report the complete genome of Paenibacillus sp. CAA11, a newly isolated promising microbial host for CBP-producing ethanol and organic acids from cellulose. The genome of Paenibacillus sp. CAA11 comprises one 4,888,410 bp chromosome with a G + C content of 48.68% containing 4418 protein-coding genes, 102 tRNA genes, and 39 rRNA genes. The functionally active cellulase, encoded by CAA_GH5 was identified to belong to glycosyl hydrolase family 5 (GH5) and consisted of a catalytic domain and a cellulose-binding domain 3 (CBM3). When cellulolytic activity of CAA_GH5 was assayed through Congo red method by measuring the size of halo zone, the recombinant Bacillus subtilis RIK1285 expressing CAA_GH5 showed a comparable cellulolytic activity to B. subtilis RIK1285 expressing Cel5, a previously verified powerful bacterial cellulase. This study demonstrates the potential of Paenibacillus sp. CAA11 as a CBP-enabling microbe for cost-effective biofuels/chemicals production from lignocellulosic biomass.



This research was supported by C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT (Information & Communication Technology) & Future Planning (2016M3D3A1A01913249). The authors also appreciate further support by Korea Institute of Science and Technology (KIST) Institutional Program (2E28290).

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Gyeongtaek Gong
    • 1
  • Hyun Ju Oh
    • 1
    • 2
  • Sukhyeong Cho
    • 1
  • Seil Kim
    • 3
  • Min-Kyu Oh
    • 2
  • Youngsoon Um
    • 1
    • 4
  • Sun-Mi Lee
    • 1
    • 4
    Email author
  1. 1.Clean Energy Research Center, Korea Institute of Science and TechnologySeoulRepublic of Korea
  2. 2.Department of Chemical and Biological EngineeringKorea UniversitySeoulRepublic of Korea
  3. 3.Division of Chemical and Medical Metrology, Center for BioanalysisKorea Research Institute of Standards and ScienceDaejeonRepublic of Korea
  4. 4.Division of Energy and Environmental TechnologyUniversity of Science and TechnologyDaejeonRepublic of Korea

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