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The Genome Sequence of a Novel Cyanophage S-B64 from the Yellow Sea, China

  • Siyuan You
  • Min WangEmail author
  • Yong JiangEmail author
  • Tong Jiang
  • Yundan Liu
  • Xinxin Liu
  • Qingwei Yang
  • Hongbing Shao
Article
  • 52 Downloads

Abstract

A novel cyanophage, S-B64, which can infect marine Synechococcus WH8102, was isolated from the coastal waters of the Yellow Sea using the liquid serial dilution method. Morphological study by transmission electron microscopy revealed that the cyanophage belongs to Podovirus. It’s genome, which was completely sequenced, contains a 151,867 bp DNA molecule with a G+C content of 41.78% and 186 potential open reading frames. The functions of the genes include cyanophage structure, cyanophage packaging, DNA replication and regulation. After primary characterization, it was found that the latent period is about 3 h, and it lysed after 8 h, the burst size is about 23 virions per cell. This information will provide an important benchmark for further research on the interaction between cyanophages and their hosts.

Notes

Acknowledgments

This study was support by the fund from the National Natural Science Foundation of China (No. 31500339, 41676178 and 41076088), and the Marine Scientific and Technological Innovation Project Financially Supported by Pilot National Laboratory for Marine Science and Technology (Qingdao) (2018SDKJ0406-6, 2016ASKJ14), at the same time, thanks are due to the Fundamental Research Funds for the Central University of Ocean University of China (Grant nos. 201812002, 201762017, 201562018).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Marine Life SciencesOcean University of ChinaQingdaoChina
  2. 2.Institute of Evolution and Marine BiodiversityOcean University of ChinaQingdaoChina
  3. 3.Key Lab of Polar Oceanography and Global Ocean ChangeOcean University of ChinaQingdaoChina

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