Methane Emission, Rumen Fermentation, and Microbial Community Response to a Nitrooxy Compound in Low-Quality Forage Fed Hu Sheep

  • Fei Xie
  • Lingli Zhang
  • Wei JinEmail author
  • Zhenxiang Meng
  • Yanfen Cheng
  • Jing Wang
  • Weiyun Zhu


The effects of nitroglycerine (NG) on the rumen methane emission, fermentation, and microbial community of Hu sheep were investigated. Eight sheep were fed NG (100 mg/head/day); another eight sheep served as controls. NG decreased methane emission of Hu sheep by ~ 19.3% (P < 0.05) without adversely affecting the production performance or rumen fermentation (P > 0.05). The alpha and beta diversity indexes of the bacterial and archaeal community showed no significant differences (P > 0.05). The dominant methanogenic species was the Methanobrevibacter gottschalkii clade, accounting for ~ 60%, followed by the Methanobrevibacter boviskoreani and Methanobrevibacter ruminantium clades. Prevotella 1 was the most dominant bacterial genus, accounting for ~ 42%, followed by the Rikenellaceae RC9 and Bacteroidales BS11 gut groups. In addition, pearson correlation analysis showed a few Methanomassiliicoccales species significantly correlated with several bacterial genera (P < 0.05).



This research was supported by the National Natural Science Foundation of China (31872381), and the Fundamental Research Funds for the Central Universities (KYZ201854).

Compliance with Ethical Standards

Competing interests

The authors have declared that no competing interests exist.

Supplementary material

284_2019_1644_MOESM1_ESM.docx (279 kb)
Supplementary material 1 (DOCX 279 KB)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Fei Xie
    • 1
    • 2
  • Lingli Zhang
    • 1
    • 3
  • Wei Jin
    • 1
    • 2
    Email author
  • Zhenxiang Meng
    • 1
    • 2
  • Yanfen Cheng
    • 1
    • 2
  • Jing Wang
    • 1
    • 2
  • Weiyun Zhu
    • 1
    • 2
  1. 1.Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and TechnologyNanjing Agricultural UniversityNanjingChina
  2. 2.National Center for International Research on Animal Gut NutritionNanjing Agricultural UniversityNanjingChina
  3. 3.Shantou University Medical CollegeShantouChina

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