Tepidicella baoligensis sp. nov., A Novel Member of Betaproteobacterium Isolated from an Oil Reservoir

  • Jing You
  • Yongbin Li
  • Shan Hong
  • Jing Wang
  • Jiliang Yu
  • Bozhong MuEmail author
  • Xiaochen MaEmail author
  • Yanfen Xue


A Gram-negative, non-pigmented, aerobic bacterium, designated strain B18-50T was isolated from oil-well production water in Baolige oilfield, China. The strain was able to grow at pH 6.5–10.5 (optimum at pH 7.5–8.5), in 0–3% (w/v) NaCl (optimum at 0–0.5%, w/v) and at 20–60 °C (optimum at 45 °C). Cells of the isolate were motile with a single polar flagellum and non-spore-forming rods. Organic acids and amino acids were used as carbon and energy sources, but sugars and polyols were not assimilated. The major cellular fatty acids were C16:0, C16:1ω6c/ω7c, and C18:1ω7c. Ubiquinone 8 was the predominant respiratory quinone. The major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The genomic DNA G+C content of the isolate was 62.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain B18-50T was most closely related to Tepidicella xavieri DSM 19605T (97.5% similarity). Comparative analysis of genotypic and phenotypic features indicate that strain B18-50T represents a novel species of the genus Tepidicella, for which the name Tepidicella baoligensis sp. nov. is proposed. The type strain is B18-50T (= CGMCC 1.13575T = KCTC 62779T).



This work was supported by the China National Petroleum Corporation (Grant Number 2017E-1507) and the Huabei Oilfield Company (Grant Number 2017-HB-B19).

Compliance with Ethical Standards

Conflict of interest

The authors have declared that no competing interests exist.

Ethical approval

The authors have declared that no ethical issues exist.

Supplementary material

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  1. 1.
    Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230CrossRefGoogle Scholar
  2. 2.
    Degryse E, Glansdorff N, Pierard A (1978) A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus. Arch Microbiol 117:189–196CrossRefGoogle Scholar
  3. 3.
    De Ley J, Cattoir H, Reynaerts A (1970) The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142CrossRefGoogle Scholar
  4. 4.
    Dong XZ, Cai MY (2001) Determinative manual for routine bacteriology. Scientific, Beijing (in Chinese) Google Scholar
  5. 5.
    Embley TM (1991) The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. Lett Appl Microbiol 13:171–174CrossRefGoogle Scholar
  6. 6.
    França L, Rainey FA, Nobre MF, da Costa MS (2006) Tepidicella xavieri gen. nov., sp. nov., a betaproteobacterium isolated from a hot spring runoff. Int J Syst Evol Microbiol 56(4):907–912CrossRefGoogle Scholar
  7. 7.
    Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA, Krieg NR, Phillips GB (1981) Manual of methods for general bacteriology. American Society for Microbiology, Washington, DCGoogle Scholar
  8. 8.
    Kieft TL, Fredrickson JK, Onstott TC, Gorby YA, Kostandarithes HM, Bailey TJ, Kennedy DW, Li SW, Plymale AE, Spadoni CM, Gray MS (1999) Dissimilatory reduction of Fe(III) and other electron acceptors by a Thermus isolate. Appl Environ Microbiol 65:1214–1221PubMedPubMedCentralGoogle Scholar
  9. 9.
    Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874CrossRefGoogle Scholar
  10. 10.
    Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218CrossRefGoogle Scholar
  11. 11.
    Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118CrossRefGoogle Scholar
  12. 12.
    Minnikin DE, Collins MD, Goodfellow M (1979) Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol: 47:87–95CrossRefGoogle Scholar
  13. 13.
    Smibert RM, Krieg NR (1981) General characterization. In: Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA, Krieg NR, Phillips GB (eds) Manual of methods for general microbiology. American Society for Microbiology, Washington, DC, pp 409–443Google Scholar
  14. 14.
    Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol 67:1613–1617CrossRefGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.School of Chemistry and Molecular EngineeringEast China University of Science and TechnologyShanghaiChina
  2. 2.Institute of MicrobiologyCASBeijingChina
  3. 3.Engineering Technology Research Institute of Huabei Oilfield CompanyRenqiuChina

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