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Current Microbiology

, Volume 75, Issue 10, pp 1378–1383 | Cite as

Complete Genome Sequence of Bacillus sp. SJ-10 (KCCM 90078) Producing 400-kDa Poly-γ-glutamic Acid

  • Won Je Jang
  • Jong Min Lee
  • Yu-Ri Kim
  • Md Tawheed Hasan
  • In-Soo KongEmail author
Article

Abstract

Bacillus sp. SJ-10 (KCCM 90078, JCM 15709) is a halotolerant bacterium isolated from a traditional Korean food, i.e., salt-fermented fish (jeotgal). The bacterium can survive and engage in metabolism at high salt concentrations. Here, we reported complete genome sequence of Bacillus sp. SJ-10, which has a single circular chromosome of 4,041,649 base pairs with a guanine–cytosine content of 46.39%. Bacillus sp. SJ-10 encodes a subunit of poly-γ-glutamic acid (γ-PGA) with a molecular weight of approximately 400 kDa, which contains four γ-PGA synthases (pgsB, pgsC, pgsAA and pgsE) and one γ-PGA-releasing gene (pgsS). This bacterium also able to produce salt-stable enzymes such as protease, β-glucosidase, and β-1,3–1,4-glucanase. This affords significant insights into strategies employed by halotolerant bacteria to survive at high salt concentrations. The sequence contains information on secondary metabolites biosynthetic gene cluster, and most importantly enzymes produced by the bacterium may be valuable with respect to food, beverage, detergent, animal feed, and certain commercial contexts.

Notes

Acknowledgements

The authors gratefully acknowledge to Dr. Moo-Sang Kim (Digital Omics Co., Korea) for providing useful information on genome analysis.

Supplementary material

284_2018_1533_MOESM1_ESM.docx (516 kb)
Supplementary material 1 (DOCX 515 KB)

References

  1. 1.
    Arndt D, Grant JR, Marcu A, Sajed T, Pon A, Liang Y, Wishart DS (2016) PHASTER: a better, faster version of the PHAST phage search tool. Nucleic Acids Res 44(W1):W16–W21CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Aslam Z, Lim JH, Im WT, Yasir M, Chung YR, Lee ST (2007) Salinicoccus jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 57(3):633–638CrossRefPubMedGoogle Scholar
  3. 3.
    Barros Soares LH, Assmann F, Záchia Ayub MA (2003) Purification and properties of a transglutaminase produced by a Bacillus circulans strain isolated from the Amazon environment. Biotechnol Appl Biochem 37(3):295–299CrossRefPubMedGoogle Scholar
  4. 4.
    Boonyapakron K, Jaruwat A, Liwnaree B, Nimchua T, Champreda V, Chitnumsub P (2017) Structure-based protein engineering for thermostable and alkaliphilic enhancement of endo-β-1, 4-xylanase for applications in pulp bleaching. J Biotechnol 259:95–102CrossRefPubMedGoogle Scholar
  5. 5.
    Candela T, Fouet A (2006) Poly-gamma-glutamate in bacteria. Mol Microbiol 60(5):1091–1098CrossRefPubMedGoogle Scholar
  6. 6.
    Chen R, Zhang C, Yao B, Xue G, Yang W, Zhou X, Sun C, Chen P, Fan Y (2013) Corn seeds as bioreactors for the production of phytase in the feed industry. J Biotechnol 165(2):120–126CrossRefPubMedGoogle Scholar
  7. 7.
    Chin CS, Alexander DH, Marks P, Klammer AA, Drake J, Heiner C, Clum A, Copeland A, Huddleston J, Eichler EE, Turner SW, Korlach J (2013) Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data. Nat Methods 10(6):563–569CrossRefPubMedGoogle Scholar
  8. 8.
    Cordeiro AL, Lenk T, Werner C (2011) Immobilization of Bacillus licheniformis α-amylase onto reactive polymer films. J Biotechnol 154(4):216–221CrossRefPubMedGoogle Scholar
  9. 9.
    Fukami K, Satomi M, Funatsu Y, Kawasaki KI, Watabe S (2004) Characterization and distribution of Staphylococcus sp. implicated for improvement of fish sauce odor. Fish Sci 70(5):916–923CrossRefGoogle Scholar
  10. 10.
    Kim EY, Kim DK, Kim YR, Choi SY, Kong IS (2009) Isolation and identification of halotolerant Bacillus sp. SJ-10 and characterization of its extracellular protease. Kor J Microbiol 45(2):193–199Google Scholar
  11. 11.
    Kim YR, Kim EY, Lee JM, Kim JK, Kong IS (2013) Characterisation of a novel Bacillus sp. SJ-10 β-1, 3–1, 4-glucanase isolated from jeotgal, a traditional Korean fermented fish. Bioprocess Biosyst Eng 36(6):721–727CrossRefPubMedGoogle Scholar
  12. 12.
    Kirk O, Borchert TV, Fuglsang CC (2002) Industrial enzyme applications. Curr Opin Biotechnol 13(4):345–351CrossRefPubMedGoogle Scholar
  13. 13.
    Kurtz S, Phillippy A, Delcher AL, Smoot M, Shumway M, Antonescu C, Salzberg SL (2004) Versatile and open software for comparing large genomes. Genome Biol 5(2):R12CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Lee JM, Kim YR, Kim JK, Jeong GT, Ha JC, Kong IS (2015) Characterization of salt-tolerant β-glucosidase with increased thermostability under high salinity conditions from Bacillus sp. SJ-10 isolated from jeotgal, a traditional Korean fermented seafood. Bioprocess Biosyst Eng 38(7):1335–1346CrossRefPubMedGoogle Scholar
  15. 15.
    Lee JM, Kim JH, Kim KW, Lee BJ, Kim DG, Kim YO, Lee JH, Kong IS (2017) Physicochemical properties, production, and biological functionality of poly-γ-D-glutamic acid with constant molecular weight from halotolerant Bacillus sp. SJ-10. Int J Biol Macromol 108:598–607CrossRefPubMedGoogle Scholar
  16. 16.
    Margesin R, Palma N, Knauseder F, Schinner F (1992) Purification and characterization of an alkaline serine protease produced by a psychrotrophic Bacillus sp. J Biotechnol 24(2):203–206CrossRefGoogle Scholar
  17. 17.
    Mark SS, Crusberg TC, DaCunha CM, Iorio AAD (2006) A heavy metal biotrap for wastewater remediation using poly-γ-glutamic acid. Biotechnol Prog 22(2):523–531CrossRefPubMedGoogle Scholar
  18. 18.
    Priest FG (1977) Extracellular enzyme synthesis in the genus Bacillus. Bacteriol Rev 41(3):711PubMedPubMedCentralGoogle Scholar
  19. 19.
    Singer JW (2005) Paclitaxel poliglumex (XYOTAX™, CT-2103): a macromolecular taxane. J Control Release 109(1):120–126CrossRefPubMedGoogle Scholar
  20. 20.
    Tanimoto H, Mori M, Motoki M, Torii K, Kadowaki M, Noguchi T (2001) Natto mucilage containing poly-γ-glutamic acid increases soluble calcium in the rat small intestine. Biosci Biotechnol Biochem 65(3):516–521CrossRefPubMedGoogle Scholar
  21. 21.
    Varela-Álvarez E, Andreakis N, Lago-Lestón A, Pearson GA, Serrao EA, Procaccini G, Duarte CM, Marba N (2006) Genomic DNA isolation from green and brown algae (Caulerpales and Fucales) for microsatellite library construction. J Phycol 42(3):741–745CrossRefGoogle Scholar
  22. 22.
    Wei KSC, Teoh TC, Koshy P, Salmah I, Zainudin A (2015) Cloning, expression and characterization of the endoglucanase gene from Bacillus subtilis UMC7 isolated from the gut of the indigenous termite Macrotermes malaccensis in Escherichia coli. Electron J Biotechnol 18(2):103–109CrossRefGoogle Scholar
  23. 23.
    Zhuge B, Du GC, Shen W, Zhuge J, Chen J (2008) Expression of a Bacillus subtilis pectate lyase gene in Pichia pastoris. Biochem Eng J 40(1):92–98CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Won Je Jang
    • 1
  • Jong Min Lee
    • 2
  • Yu-Ri Kim
    • 1
    • 3
  • Md Tawheed Hasan
    • 1
  • In-Soo Kong
    • 1
    Email author
  1. 1.Department of BiotechnologyPukyong National UniversityBusanRepublic of Korea
  2. 2.Industrial Bio-Materials Research CenterKorea Research Institute of Bioscience and BiotechnologyDaejeonRepublic of Korea
  3. 3.CBR Defense Research InstituteSeoulRepublic of Korea

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