Current Microbiology

, Volume 75, Issue 10, pp 1378–1383 | Cite as

Complete Genome Sequence of Bacillus sp. SJ-10 (KCCM 90078) Producing 400-kDa Poly-γ-glutamic Acid

  • Won Je Jang
  • Jong Min Lee
  • Yu-Ri Kim
  • Md Tawheed Hasan
  • In-Soo KongEmail author


Bacillus sp. SJ-10 (KCCM 90078, JCM 15709) is a halotolerant bacterium isolated from a traditional Korean food, i.e., salt-fermented fish (jeotgal). The bacterium can survive and engage in metabolism at high salt concentrations. Here, we reported complete genome sequence of Bacillus sp. SJ-10, which has a single circular chromosome of 4,041,649 base pairs with a guanine–cytosine content of 46.39%. Bacillus sp. SJ-10 encodes a subunit of poly-γ-glutamic acid (γ-PGA) with a molecular weight of approximately 400 kDa, which contains four γ-PGA synthases (pgsB, pgsC, pgsAA and pgsE) and one γ-PGA-releasing gene (pgsS). This bacterium also able to produce salt-stable enzymes such as protease, β-glucosidase, and β-1,3–1,4-glucanase. This affords significant insights into strategies employed by halotolerant bacteria to survive at high salt concentrations. The sequence contains information on secondary metabolites biosynthetic gene cluster, and most importantly enzymes produced by the bacterium may be valuable with respect to food, beverage, detergent, animal feed, and certain commercial contexts.



The authors gratefully acknowledge to Dr. Moo-Sang Kim (Digital Omics Co., Korea) for providing useful information on genome analysis.

Supplementary material

284_2018_1533_MOESM1_ESM.docx (516 kb)
Supplementary material 1 (DOCX 515 KB)


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Won Je Jang
    • 1
  • Jong Min Lee
    • 2
  • Yu-Ri Kim
    • 1
    • 3
  • Md Tawheed Hasan
    • 1
  • In-Soo Kong
    • 1
    Email author
  1. 1.Department of BiotechnologyPukyong National UniversityBusanRepublic of Korea
  2. 2.Industrial Bio-Materials Research CenterKorea Research Institute of Bioscience and BiotechnologyDaejeonRepublic of Korea
  3. 3.CBR Defense Research InstituteSeoulRepublic of Korea

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