Dokdonia ponticola sp. nov., A Carrageenan-Degrading Bacterium of the Family Flavobacteriaceae Isolated from Seawater
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A Gram-stain-negative, aerobic, non-motile, and carrageenan-degrading bacterial strain, designated OISW-17T, was isolated from seawater around Oido, an island of South Korea, and its taxonomic position was determined using a polyphasic study. Optimal growth of the novel strain occurred at 20–25 °C and in the presence of 2.0% (w/v) NaCl. Phylogenetic trees using 16S rRNA gene sequences showed that strain OISW-17T forms a cluster with the type strains of Dokdonia species. The novel strain exhibited 16S rRNA gene sequence similarities of 95.7–96.3% to the type strains of Dokdonia species and of < 93.4% to the type strains of the other recognized species. Menaquinone-6 (MK-6) was found as the predominant menaquinone and iso-C15:0, iso-C15:1 G, and iso-C17:0 3-OH were the major fatty acids. Phosphatidylethanolamine, one unidentified lipid, and one unidentified aminolipid were major polar lipids detected in strain OISW-17T. Strain OISW-17T had DNA G+C content of 39.7 mol%. The differential phenotypic characteristics, along with the phylogenetic data, made strain OISW-17T to be distinct from recognized species of the genus Dokdonia. On the basis of the data given, strain OISW-17T represents a novel species of the genus Dokdonia, for which the name Dokdonia ponticola sp. nov. is proposed. The type strain of the novel species is OISW-17T (= KACC 19437T = KCTC 62187T = CGMCC 1.16527T).
This work was supported by the project on survey of indigenous species of Korea of the National Institute of Biological Resources (NIBR) under the Ministry of Environment (MOE) and “Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ012956)” of Rural Development Administration, the Republic of Korea.
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Authors declare that they have no conflict of interest.
Research Involving Human and Animal Participants
This article does not contain any studies with human participants or animals performed by any of the authors.
- 2.Baumann P, Baumann L (1981) The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In: Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds) The prokaryotes. Springer, Berlin, pp 1302–1331Google Scholar
- 3.Bernardet JF (2011) Family I. Flavobacteriaceae Reichenbach 1992. In: Krieg NR, Staley JT, Brown DR, Hedlund BP, Paster BJ, Staley JT, Ward N, Brown D, Parte A (eds) Bergey’s manual of systematic bacteriology, vol 4, 2nd edn. Springer, New York, pp 106–111Google Scholar
- 10.Embley TM, Wait R (1994) Structural lipids of eubacteria. In: Goodfellow M, O’Donnell AG (eds) Modern microbial methods. Chemical methods in prokaryotic systematics. Wiley, Chichester, pp 121–161Google Scholar
- 14.Lányí B (1987) Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67Google Scholar
- 17.Reichenbach H (1992) The order Cytophagales. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH (eds) The prokaryotes. A handbook on the biology of bacteria: ecophysiology, isolation, identification, applications, 2nd edn. Springer, New York, pp 3631–3675Google Scholar
- 18.Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. MIDI Inc, Newark, DEGoogle Scholar
- 23.Yoon JH, Kang SJ, Park S, Oh TK (2012) Reclassification of the three species of the genus Krokinobacter into the genus Dokdonia as Dokdonia genika comb. nov. Dokdonia diaphoros comb. nov. and Dokdonia eikasta comb. nov., and emended description of the genus Dokdonia Yoon et al. 2005. Int J Syst Evol Microbiol 62:1896–1901CrossRefPubMedGoogle Scholar