Current Microbiology

, Volume 75, Issue 8, pp 1103–1107 | Cite as

iTRAQ-Based Proteomic Analysis of Wheat Bunt Fungi Tilletia controversa, T. caries, and T. foetida

  • Chao Li
  • Xiaoqing Wei
  • Li GaoEmail author
  • Wanquan ChenEmail author
  • Taiguo Liu
  • Bo Liu


This is the first study of proteomics of wheat bunt fungi Tilletia controversa (TCK), T. caries (TCT), and T. foetida (TFL) using the iTRAQ technique. Based on the relative quantities of specific proteins between each two pathogens, we found 50 up-regulated and 80 down-regulated protein genes in TCK compared to TFL, 62 up-regulated and 82 down-regulated protein genes in TCT compared to TFL, 47 up-regulated and 30 down-regulated protein genes in TCK compared to TCT, and there were 1 protein of up-regulated and 4 proteins of down-regulated in the three pairs. These protein data could be of great value for exploring the key proteins which play an important role in the interactions of these pathogens with their host. Some of them could be valuable for differentiating the three pathogens with monoclonal antibodies produced by the specific proteins and may enable in-site detection of the pathogens and performing routine monitoring as a diagnostic assay in wheat shipments.



This work was supported by NSFC-CGIAR (31761143011), National Key R & D Program of China (2016YFC1201200), National Natural Science Foundation (31571965), Natural Science Foundation of Beijing (6162022), and Outstanding Young Guide Fund of CAAS (Y2017JC09) and Chinese Ministry of Agriculture (CARS-03).

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflicts of interests.

Supplementary material

284_2018_1490_MOESM1_ESM.pptx (3.9 mb)
Supplementary material 1 (PPTX 4022 KB)
284_2018_1490_MOESM2_ESM.xlsx (89 kb)
Supplementary material 2. Table S1: Up and down-regulated proteins in TCK compared to TFL (XLSX 88 KB)
284_2018_1490_MOESM3_ESM.xlsx (97 kb)
Supplementary material 3. Table S2: Up and down-regulated proteins in TCT compared to TFL (XLSX 97 KB)
284_2018_1490_MOESM4_ESM.xlsx (60 kb)
Supplementary material 4. Table S3: Up and down-regulated proteins in TCK compared to TCT (XLSX 59 KB)
284_2018_1490_MOESM5_ESM.xlsx (14 kb)
Supplementary material 5. Table S4: Up and down-regulated proteins in the three pairs (XLSX 13 KB)
284_2018_1490_MOESM6_ESM.docx (11 kb)
Supplementary material 6 (DOCX 11 KB)


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina
  2. 2.College of Plant ProtectionGansu Agricultural UniversityLanzhouChina

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