SOS Response Inhibitory Properties by Potential Probiotic Formulations of Bacillus amyloliquefaciens B-1895 and Bacillus subtilis KATMIRA1933 Obtained by Solid-State Fermentation
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The ability of fermentates of two potential probiotic strains, Bacillus amyloliquefaciens B-1895 and Bacillus subtilis KATMIRA1933, to lower the SOS response in bacteria was evaluated using Escherichia coli-based Lux biosensors (pRecA-lux) and the tested bacilli fermentates obtained through solid-state fermentation. The SOS response was stimulated by the addition of ciprofloxacine. Preparations of both Bacillus fermentates demonstrated SOS-inhibitory activity (up to 54.21%). The strain КATMIRA1933 was characterized by higher SOS-inhibitory activity. The active components of the fermentates were stable against heating, proteinase, and RNase action.
This work was supported by Russian Science Foundation (Project No. 16-16-04032).
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Conflict of interest
The authors declare no conflict of interests.
- 4.Chistyakov VA, Prazdnova EV, Kharchenko EY, Kurbatov SV, Batiushin MM, Levitskaya ES, Mazanko MS, Churilov MN (2016) 7-(1-Methyl-3-Pyrrolyl-)-4, 6-dinitrobenzofuroxan reduces the frequency of antibiotic resistance mutations induced by ciprofloxacin in bacteria. Int J BioMed 6(3):228–232CrossRefGoogle Scholar
- 15.Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, New YorkGoogle Scholar
- 18.Park GH, Song HM, Kim YS, Jeon Y, Koo JS, Jeong HJ, Jeong JB (2017) Anti-cancer activity of Bacillus amyloliquefaciens AK-0 through cyclin D1 proteasomal degradation via GSK3β-dependent phosphorylation of threonine-286. Pharmazie 72(6):348–354Google Scholar
- 24.Sengupta S, Bandyopadhyay S (2012) De novo design of potential RecA inhibitors using multi objective optimization. TCBB 9(4):1139–1154Google Scholar
- 25.Syngai GG, Gopi R, Bharali R, Dey S, Lakshmanan GMA, Ahmed G (2016) Probiotics—the versatile functional food ingredients. JFST 53(2):921–933Google Scholar
- 27.Walker GC (1984) Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiol Rev 48(1):60–93Google Scholar