Current Microbiology

, Volume 75, Issue 1, pp 40–48 | Cite as

Extracellular Expression in Aspergillus niger of an Antibody Fused to Leishmania sp. Antigens

  • Denis Magaña-Ortíz
  • Francisco Fernández
  • Achim M. Loske
  • Miguel A. Gómez-Lim
Article

Abstract

Nucleoside hydrolase and sterol 24-c-methyltransferase, two antigenic proteins of Leishmania sp., were expressed in Aspergillus niger. Genetic transformation of conidia was achieved using underwater shock waves. scFv antibody addressed to DEC205, a receptor of dendritic cells, was fused to two proteins of Leishmania sp. Receptor 205 has a relevant role in the immune system in mammals; it can modulate T cell response to different antigens. Extracellular expression strategy of recombinant antibody was achieved using a fragment of native glucoamylase A (514 aa) as a carrier. Fermentations in shake flasks showed that the recombinant protein (104 kDa) was expressed and secreted only when maltose was used as carbon source; on the contrary, the expression was highly repressed in presence of xylose. Noteworthy, recombinant protein was secreted without glucoamylase-carrier and accumulation at intracellular level was not observed. The results presented here demonstrate the high value of Aspergillus niger as biotechnological platform for recombinant antibodies against Leishmania sp. at low cost. To the best of our knowledge, this is the first report about the recombinant expression of antigenic proteins of Leishmania sp. in filamentous fungi. The protein obtained can be used to explore novel strategies to induce immunity against Leishmania sp. or it can be employed in diagnostic kits to detect this neglected disease.

Notes

Acknowledgements

The authors would like to thank Ma. Concepción Arredondo, René Preza and Guillermo Vázquez for technical assistance. D.M.O. gratefully acknowledges the support of a Ph.D. scholarship (Number 219950) from CONACYT.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Denis Magaña-Ortíz
    • 1
  • Francisco Fernández
    • 2
  • Achim M. Loske
    • 2
  • Miguel A. Gómez-Lim
    • 3
  1. 1.División de Estudios de Posgrado e InvestigaciónInstituto Tecnológico de MéridaMéridaMexico
  2. 2.Centro de Física Aplicada y Tecnología AvanzadaUniversidad Nacional Autónoma de MéxicoQuerétaroMexico
  3. 3.Departamento de Ingeniería GenéticaCINVESTAV IrapuatoIrapuatoMexico

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