Advertisement

Current Microbiology

, Volume 74, Issue 3, pp 320–324 | Cite as

Biodegradation of Aldrin and Dieldrin by the White-Rot Fungus Pleurotus ostreatus

  • Adi Setyo Purnomo
  • Refdinal Nawfa
  • Fahimah Martak
  • Kuniyoshi Shimizu
  • Ichiro Kamei
Article

Abstract

Aldrin and its metabolite dieldrin are persistent organic pollutants that contaminate soil in many parts of the world. Given the potential hazards associated with these pollutants, an efficient degradation method is required. In this study, we investigated the ability of Pleurotus ostreatus to transform aldrin as well as dieldrin in pure liquid cultures. This fungus completely eliminated aldrin in potato dextrose broth (PDB) medium during a 14-day incubation period. Dieldrin was detected as the main metabolite, and 9-hydroxylaldrin and 9-hydroxyldieldrin were less abundant metabolites. The proposed route of aldrin biotransformation is initial metabolism by epoxidation, followed by hydroxylation. The fungus was also capable of degrading dieldrin, a recalcitrant metabolite of aldrin. Approximately 3, 9, and 18% of dieldrin were eliminated by P. ostreatus in low-nitrogen, high-nitrogen, and PDB media, respectively, during a 14-day incubation period. 9-Dihydroxydieldrin was detected as a metabolite in the PDB culture, suggesting that the hydroxylation reaction occurred in the epoxide ring. These results indicate that P. ostreatus has potential applications in the transformation of aldrin as well as dieldrin.

Keywords

Polylactic Acid Dieldrin Heptachlor Aldrin Potato Dextrose Broth 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work was supported by a grant from the Research Project for International Research Collaboration and Scientific Publication 2016 No: 078/SP2H/LT/DRPM/II/2016, from the Directorate of Research and Community Service, Directorate General of Strengthening Research and Development, Ministry of Research, Technology and Higher Education, Indonesia.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Bezalel L, Hadar Y, Fu PP, Freeman JP, Cerniglia CE (1996) Initial oxidation products in the metabolism of pyrene, anthracene, fluorene, and dibenzothiophene by the white rot fungus Pleurotus ostreatus. Appl Environ Microbiol 62:2554–2559PubMedPubMedCentralGoogle Scholar
  2. 2.
    Bezalel L, Hadar Y, Cerniglia CE (1997) Enzymatic mechanisms involved in phenanthrene degradation by the white rot fungus Pleurotus ostreatus. Appl Environ Microbiol 63:2495–2501PubMedPubMedCentralGoogle Scholar
  3. 3.
    Bumpus JA, Aust SD (1987) Biodegradation of DDT [1,1,1-trichloro-2,2-bis (4-Chlorophenyl) ethane] by the white rot fungus Phanerochaete chrysosporium. Appl Environ Microbiol 53:2001–2008PubMedPubMedCentralGoogle Scholar
  4. 4.
    Chiu SW, Ching ML, Fong KL, Moore D (1998) Spent oyster mushroom substrate performs better than many mushroom mycelia in removing the biocide pentachlorophenol. Mycolog Res 102:1553–1562.CrossRefGoogle Scholar
  5. 5.
    Hidayat A, Tachibana S (2012) Characterization of polylactic acid (PLA)/kenaf composite degradation by immobilized mycelia of Pleurotus ostreatus. Int Biodet Biodeg 71:50–54.CrossRefGoogle Scholar
  6. 6.
    Kamei I, Kondo R (2005) Biotransformation of dichloro-, trichloro-, and tetrachlorodibenzo-p-dioxin by the white-rot fungus Phlebia lindtneri. Appl Microbiol Biotechnol 68:560–566CrossRefPubMedGoogle Scholar
  7. 7.
    Kamei I, Suhara H, Kondo R (2005) Phylogenetical approach to isolation of white-rot fungi capable of degrading polychlorinated dibenzo-p-dioxin. Appl Microbiol Biotechnol 69:358–366CrossRefPubMedGoogle Scholar
  8. 8.
    Kamei I, Takagi K, Kondo R (2010) Bioconversion of dieldrin by wood-rotting fungi and metabolite detection. Pest Manag Sci 66:888–891PubMedGoogle Scholar
  9. 9.
    Kasai N, Ikushiro S, Shinkyo R, Yasuda K, Hirosue S, Arisawa A, Ichinose H, Wariishi H, Sakaki T (2010) Metabolism of mono- and dichloro-dibenzo-p-dioxins by Phanerochaete chrysosporium cytochromes P450. Appl Microbiol Biotechnol 86:773–780CrossRefPubMedGoogle Scholar
  10. 10.
    Moeder M, Cajthaml T, Koeller G, Erbanová P, Šašek V (2005) Structure selectivity in degradation and translocation of polychlorinated biphenyls (Delor 103) with a Pleurotus ostreatus (oyster mushroom) culture. Chemosphere 61:370–1378CrossRefGoogle Scholar
  11. 11.
    Motomura M, Toyomasu T, Mizuno K, Shinozawa T (2003) Purification and characterization of an aflatoxin degradation enzyme from Pleurotus ostreatus. Microbiol Res 158:237–242CrossRefPubMedGoogle Scholar
  12. 12.
    Nagami H (1997) Dieldrin and chlordane residue in agriculture fields. Bull Environ Contam Toxicol 59:383–388CrossRefPubMedGoogle Scholar
  13. 13.
    Nakamura R, Kondo R, Shen MH, Ochiai H, Hisamatsu S, Sonoki S (2012) Identification of cytochrome P450 monooxygenase genes from the white-rot fungus Phlebia brevispora. AMB Express 2:8CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Nwachukwu EO, Osuji JO (2007) Bioremedial degradation of some herbicides by indigenous white rot fungus, Lentinus subnudus. J Plant Sci 2:619–624CrossRefGoogle Scholar
  15. 15.
    Palmieri G, Cennamo G, Sannia G (2005) Remazol Brilliant Blue R decolourisation by the fungus Pleurotus ostreatus and its oxidative enzymatic system. Enzyme Microbiol Technol 36:17–24.CrossRefGoogle Scholar
  16. 16.
    Pointing S (2001) Feasibility of bioremediation by white-rot fungi. Appl Microbiol Biotechnol 57:20–33CrossRefPubMedGoogle Scholar
  17. 17.
    Purnomo AS, Kamei I, Kondo R (2008) Degradation of 1,1,1-trichlro-2,2-bis (4-chlorophenyl) ethane (DDT) by brown-rot fungi. J Biosci Bioeng 105(6):614–621CrossRefPubMedGoogle Scholar
  18. 18.
    Purnomo AS, Koyama F, Mori T, Kondo R (2010) DDT degradation potential of cattle manure compost. Chemosphere 80(6):619–624CrossRefPubMedGoogle Scholar
  19. 19.
    Purnomo AS, Mori T, Kamei I, Nishii T, Kondo R (2010) Application of mushroom waste medium from Pleurotus ostreatus for bioremediation of DDT-contaminated soil. Int Biodet Biodeg 64(5):397–402CrossRefGoogle Scholar
  20. 20.
    Purnomo AS, Mori T, Kondo R (2010) Involvement of Fenton reaction in DDT degradation by brown-rot fungi. Int Biodet Biodeg 64(7):560–565CrossRefGoogle Scholar
  21. 21.
    Purnomo AS, Mori T, Takagi K, Kondo R (2011) Bioremediation of DDT contaminated soil using brown-rot fungi. Int Biodet Biodeg 65(5):691–695CrossRefGoogle Scholar
  22. 22.
    Purnomo AS, Mori T, Kamei I, Kondo R (2011) Basic studies and applications on bioremediation of DDT: a review. Int Biodet Biodeg 65(7):921–930CrossRefGoogle Scholar
  23. 23.
    Purnomo AS, Mori T, Putra SR, Kondo R (2013) Biotransformation of heptachlor and heptachlor epoxide by white-rot fungus Pleurotus ostreatus. Int Biodet Biodeg 82:40–44CrossRefGoogle Scholar
  24. 24.
    Purnomo AS, Putra SR, Shimizu K, Kondo R (2014) Biodegradation of heptachlor and heptachlor epoxide-contaminated soils by white-rot fungal inocula. Environ Sci Pollut Res 21:11305–11312CrossRefGoogle Scholar
  25. 25.
    Rigas F, Dritsa V, Marchant R, Papadopoulou K, Avramides EJ, Hatzianestis I (2005) Biodegradation of lindane by Pleurotus ostreatus via central composite design. Environ Int 31:191–196CrossRefPubMedGoogle Scholar
  26. 26.
    Rigas F, Papadopoulou K, Philippoussis A, Papadopoulou M, Chatzipavlidis J (2009) Bioremediation of lindane contaminated soil by Pleurotus ostreatus in non sterile conditions using multilevel factorial design. Water Air Soil Pollut 197:21–129CrossRefGoogle Scholar
  27. 27.
    Rozen NG, Chefetz B, Ari JB, Geva J, Hadar Y (2011) Transformation of the recalcitrant pharmaceutical compound carbamazepine by Pleurotus ostreatus: role of cytochrome P450 monooxygenase and manganese peroxidase. Environ Sci Technol 45(16):6800–6805CrossRefGoogle Scholar
  28. 28.
    Schützendübel A, Majcherczyk A, Johannes C, Hüttermann A (1999) Degradation of fluorene, anthracene, phenanthrene, fluoranthene, and pyrene lacks connection to the production of extracellular enzymes by Pleurotus ostreatus and Bjerkandera adusta. Int Biodet Biodeg 43:93–100CrossRefGoogle Scholar
  29. 29.
    Tien M, Kirk TK (1988) Lignin peroxidase of Phanerochaete chrysosporium. Methods Enzymol 161:238–249CrossRefGoogle Scholar
  30. 30.
    Xiao P, Mori T, Kamei I, Kiyota H, Takagi K, Kondo R (2011) Novel metabolic pathways of organochlorine pesticide dieldrin and aldrin by white rot fungi of the genus Phlebia. Chemosphere 85(2):218–224CrossRefPubMedGoogle Scholar
  31. 31.
    Zhao X, Hardin IR (2007) HPLC and spectrophotometric analysis of biodegradation of azo dyes by Pleurotus ostreatus. Dyes Pigm 73:322–325CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of Chemistry, Faculty of Mathematics and Natural SciencesInstitut Teknologi Sepuluh Nopember (ITS)SurabayaIndonesia
  2. 2.Department of Agro-environmental Sciences, Faculty of AgricultureKyushu UniversityFukuokaJapan
  3. 3.Department of Forest and Environmental Sciences, Faculty of AgricultureUniversity of MiyazakiMiyazakiJapan

Personalised recommendations