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Current Microbiology

, Volume 74, Issue 1, pp 84–89 | Cite as

Phytotoxic, Antifungal and Immunosuppressive Metabolites from Aspergillus terreus QT122 Isolated from the Gut of Dragonfly

  • Yi-Hui Lu
  • Li-Ping Jin
  • Li-Chun Kong
  • Ying-Lao ZhangEmail author
Article

Abstract

Insect gut microbes have been considered as a resource for bioactive metabolites. The aim of this study was to characterize the compounds of a fungus Aspergillus terreus QT122 associated with the gut of dragonfly. Five main phytotoxic, antifungal, and immunosuppressive substances were isolated from the fungus QT122. The structures of such compounds were identified as emodin (1), 1-methyl emodin (2), terrein (3), methyl 6-acetyl-4-methoxy-7,8-dihydroxynaphthalene-2-carboxylate (4), and dihydrogeodin (5) on the basis of spectroscopic analysis and by comparison of the corresponding data to those reported in the literature previously. The compound 3 exhibited the best phytotoxic activity against the radicle growth of A. retroflexus L. and E. crusgalli L. with their IC50 values of 11.2 and 3.1 μg/mL, which were comparable to that of the positive control of 2,4-dichlorophenoxyacetic acid (2,4-D) with the IC50 values of 8.1 and 1.6 μg/mL, respectively. The compounds 2–3 showed potent antifungal activity in the growth of Alternaria solani with the IC50 value of less than 0.1 μg/mL and the compound 2 also had great inhibitory effect against the growth of Fusarium oxysporum f. sp. cucumerinum (IC50 < 0.1 μg/mL), which was comparable to that of referenced cycloheximide with IC50 value of below 0.1 μg/mL. The compounds 3–5 exhibited strong immunosuppressive activities against the T cell viability with the inhibition rates of more than 99%, which were comparable to positive cyclosporin A under the concentration of 20 μM. These results suggest that the compounds 2–5 have the potential to be used as bio-control agents in agriculture or immunosuppressive agents.

Keywords

Antifungal Activity Graminearum Fermentation Broth Emodin Ethyl Acetate Extract 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work was co-financed by the National Natural Science Foundation of China (NSFC) (21002092 and 21272215) and Zhejiang Provincial Natural Science Foundation of China (LY17C010002).

Compliance with Ethical Standards

Conflict of interest

The authors declare no competing financial interest.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Yi-Hui Lu
    • 1
  • Li-Ping Jin
    • 1
  • Li-Chun Kong
    • 1
  • Ying-Lao Zhang
    • 1
    Email author
  1. 1.College of Chemistry and Life SciencesZhejiang Normal UniversityJinhuaPeople’s Republic of China

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