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Current Microbiology

, Volume 74, Issue 1, pp 42–48 | Cite as

Rhizobium albus sp. nov., Isolated from Lake Water in Xiamen, Fujian Province of China

  • Yi Li
  • Xueqian Lei
  • Yanting Xu
  • Hong Zhu
  • Meiying Xu
  • Lijun Fu
  • Wei Zheng
  • Jinli Zhang
  • Tianling Zheng
Article

Abstract

A Gram-stain-negative, aerobic bacterial strain, designated Y21T, was isolated from surface lake water in Xiamen, Fujian Province of China. Growth was observed at temperatures from 4 to 37 °C, at salinities from 0 to 7.0 % and at pH from 6.0 to 10.0. Optimum growth was observed at 28 °C, at pH 7.0 and with 1.5–2.0 % (w/v) NaCl. The highest similarity of 16S rRNA gene sequence between strain Y21T and the other strains was 96.9 %. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the strain was a member of the genus Rhizobium, forming a distinct lineage with R. subbaraonis KCTC 23614T. The dominant fatty acids were summed feature 8 (comprising C18:1 ω7c and/or C18:1 ω6c), C18:1 ω7c 11-methyl, which accounted for 78.1 %. The G+C content of the chromosomal DNA was 60.9 mol%. The predominant respiratory quinone was ubiquinone-10. The polar lipids of strain Y21T were found to consist of five unidentified phospholipids and three unidentified aminolipids. According to its morphology, physiology, fatty acid composition and 16S rRNA sequence data, strain Y21T should be regarded as a new species of the genus Rhizobium, for which Rhizobium albus sp. nov. is proposed (type strain Y21T = MCCC 1F01210T = KCTC 42252T).

Keywords

Rhizobium Respiratory Quinone Unidentified Phospholipid Surface Lake Water Unidentified Aminolipids 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

MCCC

Marine Culture Collection of China

KCTC

Korean Collection For Type Cultures

Notes

Acknowledgments

This work was supported by the NSFC (41576109, 31500095 and 31400318), the Doctoral Scientific Research Start-up Foundation of Henan Normal University (5101049170160), WEL Visiting Fellowship Program and the Key Scientific Research Programs of Henan Education Department (17A610002). We also thank Prof. I. J. Hodgkiss of The University of Hong Kong for comments and help with the English.

Compliance with Ethical Standards

Conflict of Interest

We confirm that the manuscript has been read and approved by all named authors and that there are no other persons who satisfied the criteria for authorship but are not listed. We further confirm that the order of authors listed in the manuscript has been approved by all of us. There are no conflict of interest associated with this publication.

Supplementary material

284_2016_1149_MOESM1_ESM.docx (1.3 mb)
Supplementary material 1 (DOCX 1338 kb)

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Yi Li
    • 1
  • Xueqian Lei
    • 2
  • Yanting Xu
    • 1
  • Hong Zhu
    • 2
  • Meiying Xu
    • 3
  • Lijun Fu
    • 5
  • Wei Zheng
    • 2
  • Jinli Zhang
    • 4
    • 6
  • Tianling Zheng
    • 2
  1. 1.College of Life SciencesHenan Normal UniversityXinxiangChina
  2. 2.Key Lab of the Ministry of Education for Coastal and Wetland Ecosystem, School of Life SciencesXiamen UniversityXiamenChina
  3. 3.Guangdong Institute of MicrobiologyGuangzhouChina
  4. 4.College of Food and Biological EngineeringJimei UniversityXiamenChina
  5. 5.College of Environment and Life SciencePutian UniversityPutianChina
  6. 6.Huang Chaoyang Building, Xiang’ an CampusXiamen UniversityXiamenChina

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