Current Microbiology

, Volume 73, Issue 4, pp 550–560 | Cite as

First Evidence of Altererythrobacter sp. LY02 with Indirect Algicidal Activity on the Toxic Dinoflagellate, Alexandrium tamarense

Article

Abstract

Alexandrium tamarense is a toxic harmful algal blooms (HABs) causing species, which poses great threat to human health and marine economy. In this study, we isolated an algicidal bacterium Altererythrobacter sp. LY02 towards to A. tamarense and later investigated the algicidal activity, algicidal mode, characteristics of algicidal active substance and algicidal procedure. The results indicated that the cell-free filtrate of strain LY02 showed high algicidal effect on algal growth, however, bacterial cells almost lost algicidal activity. The algicidal active substance was temperature- and pH-stability, and its molecular weight was less than 1000 Da, and was a non-proteinaceous material or non-polysaccharide, mid-polar substance. Under the algicidal effect of active substance, the morphology and structure of A. tamarense cells were seriously damaged as well as organelles. Our study confirmed that the algicidal active substance could be used as an excellent bio-agent for controlling HABs caused by A. tamarense.

References

  1. 1.
    Anderson DM (1997) Turning back the harmful red tide. Nature 388:513–514CrossRefGoogle Scholar
  2. 2.
    Anderson DM, Glibert PM, Burkholder JM (2002) Harmful algal blooms and eutrophication: nutrient sources, composition, and consequences. Estuaries 25:704–726CrossRefGoogle Scholar
  3. 3.
    Asakawa M, Miyazawa K, Takayama H, Noguchi T (1995) Dinoflagellate Alexandrium tamarense as the source of paralytic shellfish poison (PSP) contained in bivalves from Hiroshima Bay, Hiroshima Prefecture, Japan. Toxicon 33:691–697CrossRefPubMedGoogle Scholar
  4. 4.
    Franks P, Anderson D (1992) Alongshore transport of a toxic phytoplankton bloom in a buoyancy current: Alexandrium tamarense in the Gulf of Maine. Mar Biol 112:153–164CrossRefGoogle Scholar
  5. 5.
    Liang L, Gang P (2013) A universal method for flocculating harmful algal blooms in marine and fresh waters using modified sand. Environ Sci Technol 47:4555–4562CrossRefGoogle Scholar
  6. 6.
    Elder JF, Horne AJ (1978) Copper cycles and CuSO4 algicidal capacity in two California lakes. Environ Manage 2:17–30CrossRefGoogle Scholar
  7. 7.
    Zhou LH, Zheng TL, Wang X, Ye JL, Tian Y, Hong HS (2007) Effect of five chinese traditional medicines on the biological activity of a red-tide causing alga—Alexandrium tamarense. Harmful Algae 6:354–360CrossRefGoogle Scholar
  8. 8.
    Chen Z, Lei X, Zhang B, Yang L, Zhang H, Zhang J, Li Y, Zheng W, Tian Y, Liu J (2014) First report of Pseudobodo sp, a new pathogen for a potential energy-producing algae: chlorella vulgaris cultures. PLoS One 9:e89571CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Jeong HJ, Kim JS, Yoo YD, Kim ST, Song JY, Kim TH, Seong KA, Kang NS, Kim MS, Kim JH (2008) Control of the harmful alga Cochlodinium polykrikoides by the naked ciliate Strombidinopsis jeokjo in mesocosm enclosures. Harmful Algae 7:368–377CrossRefGoogle Scholar
  10. 10.
    Stocker R (2012) Marine microbes see a sea of gradients. Science 338:628–633CrossRefPubMedGoogle Scholar
  11. 11.
    Zhang B, Cai G, Wang H, Li D, Yang X, An X, Zheng X, Tian Y, Zheng W, Zheng T (2014) Streptomyces alboflavus RPS and its novel and high algicidal activity against harmful algal bloom species Phaeocystis globosa. PLoS One 9(3):e92907CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Lei X, Li Y, Chen Z, Zheng W, Lai Q, Zhang H, Guan C, Cai G, Yang X, Tian Y (2013) Altererythrobacter xiamenensis sp. nov., an algicidal bacterium isolated from red tide seawater. Int J Syst Evol Micr 64:631–637CrossRefGoogle Scholar
  13. 13.
    Guillard RR (1975) Culture of phytoplankton for feeding marine invertebrates. In: Culture of marine invertebrate animals. Springer, pp 29–60Google Scholar
  14. 14.
    Cheng Z, Ye RM, Zheng JW, Luo ZH, Gu HF, Yang WD, Li HY, Liu JS (2014) Molecular phylogeny and PSP toxin profile of the Alexandrium tamarense species complex along the coast of China. Mar Pollut Bull 89:209–219CrossRefGoogle Scholar
  15. 15.
    Ichimi K, Suzuki T, Ito A (2002) Variety of PSP toxin profiles in various culture strains of Alexandrium tamarense and change of toxin profile in natural A. tamarense population. J Exp Mar Biol Ecol 273:51–60CrossRefGoogle Scholar
  16. 16.
    Li Y, Wei J, Yang C, Lai Q, Chen Z, Li D, Zhang H, Tian Y, Zheng W, Zheng T (2013) Tenacibaculum xiamenense sp. nov., an algicidal bacterium isolated from coastal seawater. Int J Syst Evol Micr 63(9):3481–3486CrossRefGoogle Scholar
  17. 17.
    Li Y, Zhu H, Zhang H, Chen Z, Tian Y, Xu H, Zheng T, Zheng W (2014) Toxicity of algicidal extracts from Mangrovimonas yunxiaonensis strain LY01 on a HAB causing Alexandrium tamarense. J Hazard Mater 278:372–381CrossRefPubMedGoogle Scholar
  18. 18.
    Su JQ, Yang XR, Zheng TL, Tian Y, Jiao NZ, Cai LZ, Hong HS (2007) Isolation and characterization of a marine algicidal bacterium against the toxic dinoflagellate Alexandrium tamarense. Harmful Algae 6:799–810CrossRefGoogle Scholar
  19. 19.
    Kwon KK, Woo JH, Yang SH, Kang JH, Kang SG, Kim SJ, Sato T et al (2007) Kato CAltererythrobacter epoxidivorans gen. nov., sp. nov., an epoxide hydrolase-active, mesophilic marine bacterium isolated from cold-seep sediment, and reclassification of Erythrobacter luteolus Yoon, 2005 as Altererythrobacter luteolus comb. nov. Int J Syst Evol Micr 57:2207–2211CrossRefGoogle Scholar
  20. 20.
    Yang C, Li Y, Zhou Y, Zheng W, Tian Y, Zheng T (2012) Bacterial community dynamics during a bloom caused by Akashiwo sanguinea in the Xiamen sea area, China. Harmful Algae 20:132–141CrossRefGoogle Scholar
  21. 21.
    Yang C, Li Y, Zhou B, Zhou Y, Zheng W, Tian Y, Van Nostrand JD, Wu L, He Z, Zhou J (2015) Illumina sequencing-based analysis of free-living bacterial community dynamics during an Akashiwo sanguine bloom in Xiamen sea. China. Sci Rep 5:8476CrossRefPubMedGoogle Scholar
  22. 22.
    Guan C, Guo X, Cai G, Zhang H, Li Y, Zheng W, Zheng T (2014) Novel algicidal evidence of a bacterium Bacillus sp. LP-10 killing Phaeocystis globosa, a harmful algal bloom causing species. Biol Control 76:79–86CrossRefGoogle Scholar
  23. 23.
    Li D, Zhang H, Fu L, An X, Zhang B, Li Y, Chen Z, Zheng W, Yi L, Zheng T (2014) A novel algicide: evidence of the effect of a fatty acid compound from the marine bacterium, Vibrio sp. BS02 on the harmful dinoflagellate, Alexandrium tamarense. PLoS One 9:e91201CrossRefPubMedPubMedCentralGoogle Scholar
  24. 24.
    Li Y, Zhu H, Guan C, Zhang H, Guo J, Chen Z, Cai G, Lei X, Zheng W, Tian Y (2014) Towards molecular, physiological, and biochemical understanding of photosynthetic inhibition and oxidative stress in the toxic Alexandrium tamarense induced by a marine bacterium. Appl Microbiol Biot 98:4637–4652CrossRefGoogle Scholar
  25. 25.
    Kang YH, Kim JD, Kim BH, Kong DS, Han MS (2005) Isolation and characterization of a bio-agent antagonistic to diatom, Stephanodiscus hantzschii. J Appl Microbiol 98:1030–1038CrossRefPubMedGoogle Scholar
  26. 26.
    Kirchman DL (2002) The ecology of Cytophaga–Flavobacteria in aquatic environment. FEMS Microbiol Ecol 2:91–100Google Scholar
  27. 27.
    Bai SJ, Huang LP, Su JQ, Tian Y, Zheng TL (2011) Algicidal efects of a nvel mrine ainomycete on the txic dnoflagellate Alexandrium tamarense. Curr Microbiol 62:774–1781CrossRefGoogle Scholar
  28. 28.
    Mayali X, Azam F (2004) Algicidal bacteria in the sea and their impact on algal blooms1. J Eukaryot Microbiol 51:139–144CrossRefPubMedGoogle Scholar
  29. 29.
    Li Y, Zhu H, Lei X, Zhang H, Cai G, Chen Z, Fu L, Xu H, Zheng T (2015) The death mechanism of the harmful algal bloom species Alexandrium tamarense induced by algicidal bacterium Deinococcus sp. Y35. Front Microbiol 6:992PubMedPubMedCentralGoogle Scholar
  30. 30.
    Zhang HJ, An XL, Zhou YY, Zhang BZ, Zhang S, Li D, Chen ZR, Li Y, Bai SJ, Lv JL, Zheng W, Tian Y, Zheng TL (2013) Effect of oxidative stress induced by Brevibacterium sp. BS01 on a HAB causing species–Alexandrium tamarense. PLoS One 8:e63018CrossRefPubMedPubMedCentralGoogle Scholar
  31. 31.
    Sakata T, Yoshikawa T, Nishitarumizu S (2011) Algicidal activity and identification of an algicidal substance produced by marine Pseudomonas sp. C55a-2. Fisheries Sci 17:2601–2607Google Scholar
  32. 32.
    Wang B, Yang X, Lu J, Zhou Y, Su J, Tian Y, Zhang J, Wang G, Zheng T (2012) A marine bacterium producing protein with algicidal activity against Alexandrium tamarense. Harmful Algae 13:83–88CrossRefGoogle Scholar
  33. 33.
    Nakashima T, Miyazaki Y, Matsuyama Y, Muraoka W, Yamaguchi K, Oda T (2006) Producing mechanism of an algicidal compound against red tide phytoplankton in a marine bacterium γ-proteobacterium. Appl Microbiol Biot 73:684–690CrossRefGoogle Scholar
  34. 34.
    Li Y, Zhu H, Lei X, Zhang H, Guan C, Chen Z, Zheng W, Xu H, Tian Y, Yu Z, Zheng T (2015) The first evidence of deinoxanthin from Deinococcus sp. Y35 with strong algicidal effect on the toxic dinoflagellate Alexandrium tamarense. J Hazard Mater 290:87–95CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Yi Li
    • 1
  • Lei Liu
    • 1
  • Yanting Xu
    • 1
  • Chengwei Guan
    • 2
    • 3
  • Xueqian Lei
    • 2
  • Wei Zheng
    • 2
  • Hailei Wang
    • 1
  • Tianling Zheng
    • 2
    • 4
  1. 1.College of Life SciencesHenan Normal UniversityXinxiangChina
  2. 2.State Key Laboratory of Marine Environmental Science, School of Life SciencesXiamen UniversityXiamenChina
  3. 3.Tobacco Science Research Institute of Jiangxi ProvinceNanchangChina
  4. 4.Xiamen UniversityXiamenChina

Personalised recommendations