Application of Differential Proteomic Analysis to Authenticate Ophiocordyceps sinensis
Ophiocordyceps sinensis (Berk.) Sacc. is one of the most well-known fungi in traditional Chinese medicine and is attracting attention because of its nutritious and medicinal properties. The present study aimed to produce a proteomic map to identify common O. sinensis proteins. The caterpillar body and stroma of O. sinensis collected from five locations and four fungal specimens of similar appearance were examined by two-dimensional electrophoresis (2-DE). Five proteins were identified using MALDI-TOF-–TOF/MS, and the 2-DE identification pattern was provided. OCS_04585 and β-lactamase domain-containing protein, the two abundant and characteristic proteins, were separated and purified using liquid-phase isoelectric focusing. The products were high-quality materials that can be used for future protein-function studies and immunoassay development.
KeywordsInternal Transcribe Spacer Protein Spot Peptide Mass Fingerprint NCBI Protein Database Total Saccharide
- 4.Jiang Y, Yao Y (2002) Anamorphic fungi related to Cordyceps sinensis. Mycosystema 22(1):161–176Google Scholar
- 14.Rafalko A, Dai S, Hancock WS, Karger BL, Hincapie M (2011) Development of a Chip/Chip/SRM platform using digital chip isoelectric focusing and LC-Chip mass spectrometry for enrichment and quantitation of low abundance protein biomarkers in human plasma. J Proteome Res 11(2):808–817PubMedCentralCrossRefPubMedGoogle Scholar