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Current Microbiology

, Volume 72, Issue 1, pp 55–63 | Cite as

Bacterial Diversity in Bohai Bay Solar Saltworks, China

  • Jiaojiao Zhang
  • Guannan Ma
  • Yuangao Deng
  • Jinggang Dong
  • Gilbert Van Stappen
  • Liying SuiEmail author
Article

Abstract

The microbiota in solar salterns plays an important role in salt production quantitatively and qualitatively. Bohai Bay coast is the major sea salt producing area in China. However, few ecological characterization studies of the Bohai Bay salt ponds, particularly of their microbial diversity, have been conducted. This study investigated the structure and diversity of the bacterial community in Hangu saltworks in response to environmental factors. The brine water was sampled from five selected saltponds within a salinity range of 5.0–19.3 % in May, July, and October, 2012. Phylogenetic analysis based on the denaturing gradient gel electrophoresis (DGGE) patterns of the PCR-amplified 16S rRNA gene fragment showed that, rather than pond salinity, especially the month of sampling influenced the structure of the bacterial community in the saltponds, which may be related to the water temperature or other factors fluctuating over the months. Moreover, canonical correspondence analysis of biological and physico-chemical parameters indicated that especially other environmental factors such as nitrogenous and phosphorous nutrient contents and pH structured the microbial community. The relatively high range-weighted richness index and Shannon–Wiener index (H′) observed in this study reflect the high level of richness and biodiversity present, though there were substantial fluctuations over the months and salinities of sampling. The fragment of 16S rRNA gene sequence recovered from DGGE bands indicated that the bacterial assemblage in Hangu Saltworks was dominated by members of γ-Proteobacteria (34 % of total sequences obtained), followed by Firmicutes (14 %) and Bacteroidetes (9 %).

Keywords

Canonical Correspondence Analysis Salinity Range Total Ammonium Nitrogen Salt Production Salt Pond 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was supported by the International Cooperation Research Program of the Ministry of Science and Technology of China (2010DFA32300), Natural Science Foundation of Tianjin City (13JCZDJC28700), Training Program for Changjiang Scholars and Innovative Research Team in University (2013-373), and the Pilot Project for International Cooperation funded by the Province of East-Flanders, Belgium.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Jiaojiao Zhang
    • 1
  • Guannan Ma
    • 1
  • Yuangao Deng
    • 1
  • Jinggang Dong
    • 1
  • Gilbert Van Stappen
    • 2
  • Liying Sui
    • 1
    Email author
  1. 1.Tianjin Key Laboratory of Marine Resources and Chemistry, College of Marine and Environmental ScienceTianjin University of Science and TechnologyTianjinChina
  2. 2.Laboratory of Aquaculture and Artemia Reference CenterGhent UniversityGhentBelgium

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