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Current Microbiology

, Volume 71, Issue 3, pp 326–332 | Cite as

Kinetics and Mechanism of Fenpropathrin Biodegradation by a Newly Isolated Pseudomonas aeruginosa sp. Strain JQ-41

  • Haihai Song
  • Zhiren Zhou
  • Yuanxiu Liu
  • Si Deng
  • Heng XuEmail author
Article

Abstract

A soil bacterium designated strain JQ-41, capable of growth on fenpropathrin as the sole carbon source and energy source, was isolated from a long-term pyrethroid insecticide-treated orchard. Based on the morphology, physio-biochemical characteristics, and 16S rDNA gene analysis, as well as the G+C content of the genomic DNA, the strain JQ-41 was identified as Pseudomonas aeruginosa. Up to 92.3 % of 50 mg l−1 fenpropathrin was degraded by P. aeruginosa strain at 30 °C and pH 7 within 7 days. The kinetic parameters q max, K s, and K i were established to be 1.14 day−1, 38.41 mg l−1, and 137.67 mg l−1, respectively, and the critical inhibitor concentration was determined to be 72.72 mg l−1. Cell surface hydrophobicity of P. aeruginosa strain was enhanced during growth on fenpropathrin. Three metabolites from fenpropathrin degradation were identified by gas chromatography mass spectrometry, and then a possible degradation pathway was proposed. In addition, this isolate was also able to degrade a wide range of synthetic pyrethroid insecticides including cypermethrin, deltamethrin, bifenthrin, and cyhalothrin with the degradation process following the first-order kinetic model. Taken together, our results provide insights into the kinetics and mechanism of fenpropathrin degradation by P. aeruginosa strain and also highlight its promising potential in bioremediation of pyrethroid-contaminated environment.

Keywords

Mineral Salt Medium Cypermethrin Deltamethrin Bifenthrin Cell Surface Hydrophobicity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was financially supported by the National High Technology Research and Development Program of China (No. 2013AA06A210), the National Natural Science Foundation of China (No. J1103518), and Chengdu Longquanyi District Science and Technology Bureau. The authors wish to thank Professor Guanglei Cheng and Dong Yu from Sichuan University for their technical assistance.

Supplementary material

284_2015_852_MOESM1_ESM.doc (612 kb)
Supplementary material 1 (DOC 612 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Haihai Song
    • 1
  • Zhiren Zhou
    • 1
  • Yuanxiu Liu
    • 1
  • Si Deng
    • 1
  • Heng Xu
    • 1
    Email author
  1. 1.Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life SciencesSichuan UniversityChengduChina

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