Current Microbiology

, Volume 70, Issue 6, pp 859–864 | Cite as

Identification of AstG1, A LAL Family Regulator that Positively Controls Ansatrienins Production in Streptomyces sp. XZQH13

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Abstract

Ansamycins is a group of type I polyketides characterized by the unique starter unit 3-amino-5-hydroxybenzoic acid. This family of secondary metabolites shows diverse biological activities, well-known members of which include rifamycin, geldanamycin, and maytansine. Previously, we isolated an AHBA synthase gene-positive strain Streptomyces sp. XZQH13 containing a “silent” ansamycin biosynthetic gene cluster ast. The constitutive expression of the Large-ATP-binding regulators of the LuxR family regulator gene astG1 located within the cluster triggered the expression of the biosynthetic genes. Reverse transcription-PCR experiments showed that the expression of the key biosynthetic genes, astB4, astD1, and astF1, was induced in the astG1 overexpression mutant compared to the wild type. This led to the isolation of two known ansatrienins, hydroxymycotrienin A (1) and thiazinotrienomycin G (2), which were identified by analysis of the mass spectral and NMR spectral data, from the mutant. These observations suggest that astG1 is probably a pathway-specific positive regulator for the biosynthesis of ansatrienin.

Keywords

Streptomyces Biosynthetic Gene Cluster Polyketide Chain Ansamycin Secondary Metabolite Biosynthetic Gene Cluster 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

This study was supported by the National Natural Science Foundation of China (31100079), 973 Programs (2012CB721005; 2010CB833802), and Program for Changjiang Scholars and Innovative Research Team in University (IRT13028).

Supplementary material

284_2015_798_MOESM1_ESM.docx (707 kb)
Supplementary material 1 (DOCX 706 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.State Key Laboratory of Microbial Technology, School of Life ScienceShandong UniversityJinanPeople’s Republic of China
  2. 2.Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical SciencesShandong UniversityJinanPeople’s Republic of China

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