Current Microbiology

, Volume 70, Issue 5, pp 716–723 | Cite as

ClpP Affects Biofilm Formation of Streptococcus mutans Differently in the Presence of Cariogenic Carbohydrates Through Regulating gtfBC and ftf

  • Jia-qin Zhang
  • Xiang-hua Hou
  • Xiu-yu Song
  • Xiao-bo Ma
  • Yuan-xun Zhao
  • Shi-yang Zhang
Article

Abstract

The abilities to form biofilms on teeth surface and to metabolize a wide range of carbohydrates are key virulence attributes of Streptococcus mutans. ClpP has been proved to play an important role in biofilm development in streptococci. Here we demonstrated that ClpP was involved in biofilm formation of S. mutans. ClpP inactivation resulted in enhanced biofilm formation or initial cell adherence in broth supplemented with sucrose, while reduced in broth supplemented with glucose or fructose. Our results also indicated that the enhanced capacities of biofilm formation and initial cell adherence were achieved through regulating the expression of a number of extracellular sucrose-metabolizing enzymes, such as glucosyltransferases (GTFB and GTFC) at early-exponential growth phase and fructosyltransferase at late-exponential growth phase in the presence of sucrose.

Notes

Acknowledgments

The authors would like to thank S. Hou for technical assistance and Q. Xu and H. Rao for critically reading the manuscript. This work was supported through funding from the National Natural Science Foundation of China (No. 81000762), the Natural Science Foundation (No. 2010D018) of Fujian Province, China.

Conflict of interest

The authors have no conflicts of interest to declare.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Jia-qin Zhang
    • 1
    • 2
  • Xiang-hua Hou
    • 3
  • Xiu-yu Song
    • 1
  • Xiao-bo Ma
    • 1
  • Yuan-xun Zhao
    • 1
  • Shi-yang Zhang
    • 2
    • 4
  1. 1.Department of Clinical LaboratoryThe First Affiliated Hospital of Xiamen UniversityXiamenChina
  2. 2.Nosocomial Infection Control Center of XiamenXiamenChina
  3. 3.Department of NephrologyThe First Affiliated Hospital of Xiamen UniversityXiamenChina
  4. 4.Department of Nosocomial Infection ControlThe First Affiliated Hospital of Xiamen UniversityXiamenChina

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