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Current Microbiology

, Volume 70, Issue 2, pp 260–266 | Cite as

Peptoniphilus mikwangii sp. nov., Isolated from a Clinical Specimen of Human Origin

  • Eugene Cho
  • Soon-Nang Park
  • Yeseul Shin
  • Yun Kyong Lim
  • Jayoung Paek
  • Hak Kyun Kim
  • Cheol Ho Hwang
  • Eojin Jo
  • Dongchun Jin
  • Young-Hyo Chang
  • Joong-Ki Kook
Article

Abstract

A novel Gram-positive, anaerobic, and coccus-shaped bacterium, designated as strain ChDC B134T, was isolated from a human postoperative infectious lesion in the right maxilla between the lateral incisor and canine and was characterized by polyphasic taxonomic analysis. 16S rRNA gene sequence analysis revealed that the strain ChDC B134T belonged to the genus Peptoniphilus, as it showed sequence similarities to Peptoniphilus indolicus KCTC 15023T (94.0 %) and Peptoniphilus asaccharolyticus KCTC 3321T (93.8 %). The prevalent fatty acids of of strain ChDC B134T were C16:0 (20.3 %), C18:1 cis 9 (34.3 %), and C18:0 (13.2 %). The DNA G+C content was 30.9 mol%. The cell wall diamino acid was d-ornithine, which is a property shared by other reference type strains of the genus Peptoniphilus. Based on the results of phenotypic, chemotaxonomic, and phylogenetic analysis, strain ChDC B134T (=KCOM 1628T = KCTC 15227T = JCM 30223T) should be classified as the type strain of a novel species of genus Peptoniphilus, for which the name Peptoniphilus mikwangii sp. nov. is proposed.

Keywords

Hemin Lateral Incisor Dentigerous Cyst Diamino Acid Cystine Arylamidase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2013M3A9B8013860) and in part by research funds from Chosun University, 2013.

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

284_2014_712_MOESM1_ESM.pptx (110 kb)
Supplementary Fig. S1. Maximum-likelihood (a) and maximum-parsimony (b) phylogenetic trees based on 16S rDNA sequences showing the relationship between strain ChDC B134T and related species. The stability of the phylogenetic trees was assessed by bootstrap analysis of 1,000 replicates using MEGA version 6.06 [25]. Bar, 0.01 changes per nucleotide position. (PPTX 110 kb)
284_2014_712_MOESM2_ESM.pptx (298 kb)
Supplementary Fig. S2. Thin-layer chromatography of acid hydrolysates of peptidoglycan. Strains: 1, strain ChDC B134T; 2, P. asaccharolyticus KCTC 3321T; 3, P. indolicus KCTC 15023T; 4, P. lacrimalis KCTC 5950T. d-orn, d-ornithine (red-colored arrow); l-orn, l-ornithine (green-colored arrow). (PPTX 298 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Eugene Cho
    • 1
  • Soon-Nang Park
    • 1
  • Yeseul Shin
    • 2
  • Yun Kyong Lim
    • 1
  • Jayoung Paek
    • 2
  • Hak Kyun Kim
    • 3
  • Cheol Ho Hwang
    • 4
  • Eojin Jo
    • 1
  • Dongchun Jin
    • 5
  • Young-Hyo Chang
    • 2
  • Joong-Ki Kook
    • 1
  1. 1.Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of DentistryChosun UniversityGwangjuRepublic of Korea
  2. 2.Korean Collection for Type Cultures, Biological Resource CenterKRIBBDaejeonRepublic of Korea
  3. 3.Department of Dentistry, College of MedicineChungnam National UniversityDeajeonRepublic of Korea
  4. 4.Department of Environmental EngineeringChosun UniversityGwangjuRepublic of Korea
  5. 5.Department of Veterinary Medicine, College of AgricultureYanbian UniversityYanjiChina

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