Current Microbiology

, Volume 70, Issue 1, pp 110–118 | Cite as

Paradevosia shaoguanensis gen. nov., sp. nov., Isolated from a Coking Wastewater

  • Shuang Geng
  • Xin-Chi Pan
  • Ran Mei
  • Ya-Nan Wang
  • Ji-Quan Sun
  • Xue-Ying Liu
  • Yue-Qin Tang
  • Xiao-Lei WuEmail author


A Gram staining negative, rod-shaped, aerobic bacterial strain J5-3T with a single polar flagellum was isolated from coking wastewater collected from Shaoguan, Guangdong, China. It was motile and capable of optimal growth at pH 6–8, 30 °C, and 0–2 % (w/v) NaCl. Its predominant fatty acids were 11-methyl C18:1 ω7c (29.2 %), C16:0 (20.6 %), C19:0 cyclo ω8c (18.2 %), C18:0 (11.0 %), and C18:1 ω7c/C18:1 ω6c (10.9 %) when grown on trypticase soy agar. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids (GL1, GL2), and two unknown phospholipid (PL1, PL2). The predominant ubiquinone was Q-10, and the genome DNA G+C content was 61.7 mol %. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain J5-3T belonged to the family Hyphomicrobiaceae in Alphaproteobacteria. It shared the 16S rRNA gene sequence similarities of 93.8–96.1 % with the genus Devosia, 94.5–94.8 % with the genus Pelagibacterium, and <92.0 % with all the other type strains in family Hyphomicrobiaceae. It can be distinguished from the closest phylogenetic neighbors based on several phenotypic and genotypic features, including α-galactosidase activity, tetracycline susceptibility, major fatty acid composition, polar lipid profile, DNA gyrase B subunit (gyrB) gene sequence, and random-amplified polymorphic DNA profile. Therefore, we consider strain J5-3T to represent a novel species of a novel genus within the family Hyphomicrobiaceae, for which the name Paradevosia shaoguanensis gen. nov., sp. nov. is proposed. The type strain of Paradevosia shaoguanensis is J5-3T (=CGMCC 1.12430T =LMG 27409T).


gyrB Gene Major Polar Lipid China General Microbiological Culture Collection Center Coke Wastewater Single Polar Flagellum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors thank the Institute of Microbiology, Chinese Academy of Sciences, for providing the transmission electron microscopy facility. This study was supported by the National Natural Science Foundation of China (31225001), and the National High Technology Research and Development Program (“863” Programs: 2012AA02A703 and 2013AA065701).

Supplementary material

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Supplementary material 1 (DOC 2082 kb)


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Shuang Geng
    • 1
  • Xin-Chi Pan
    • 1
  • Ran Mei
    • 1
  • Ya-Nan Wang
    • 2
  • Ji-Quan Sun
    • 1
    • 3
  • Xue-Ying Liu
    • 1
  • Yue-Qin Tang
    • 4
  • Xiao-Lei Wu
    • 1
    • 3
    Email author
  1. 1.College of EngineeringPeking UniversityBeijingPeople’s Republic of China
  2. 2.Institute of BiologyHenan Academy of SciencesZhengzhouPeople’s Republic of China
  3. 3.Institute of Engineering (Baotou), College of EngineeringPeking UniversityBaotouPeople’s Republic of China
  4. 4.College of Architecture and EnvironmentSichuan UniversityChengduPeople’s Republic of China

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