Current Microbiology

, Volume 69, Issue 6, pp 809–816 | Cite as

Dominance of Methanosarcinales Phylotypes and Depth-Wise Distribution of Methanogenic Community in Fresh Water Sediments of Sitka Stream from Czech Republic

  • Prem Prashant ChaudharyEmail author
  • André-Denis G. Wright
  • Lenka Brablcová
  • Iva Buriánková
  • Adam Bednařík
  • Martin Rulík


The variation in the diversity of methanogens in sediment depths from Sitka stream was studied by constructing a 16S rRNA gene library using methanogen-specific primers and a denaturing gradient gel electrophoresis (DGGE)-based approach. A total of nine different phylotypes from the 16S rRNA library were obtained, and all of them were clustered within the order Methanosarcinales. These nine phylotypes likely represent nine new species and at least 5–6 new genera. Similarly, DGGE analysis revealed an increase in the diversity of methanogens with an increase in sediment depth. These results suggest that Methanosarcinales phylotypes might be the dominant methanogens in the sediment from Sitka stream, and the diversity of methanogens increases as the depth increases. Results of the present study will help in making effective strategies to monitor the dominant methanogen phylotypes and methane emissions in the environment.


Sediment Depth Methanosarcinales Methanosaeta Methanomicrobiales Methanobacteriales 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors are thankful to the European Social Fund and state budget of the Czech Republic for providing the financial support during this study. This work is a part of the POSTUP II project CZ.1.07/2.3.00/30.0041, which is mutually financed by the previously stated funding agencies.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Prem Prashant Chaudhary
    • 1
    Email author
  • André-Denis G. Wright
    • 2
  • Lenka Brablcová
    • 1
  • Iva Buriánková
    • 1
  • Adam Bednařík
    • 1
  • Martin Rulík
    • 1
  1. 1.Laboratory of Aquatic Microbial Ecology, Department of Ecology and Environmental Sciences, Faculty of SciencePalacky UniversityOlomoucCzech Republic
  2. 2.Department of Animal ScienceUniversity of VermontBurlingtonUSA

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