Current Microbiology

, Volume 69, Issue 4, pp 495–500 | Cite as

Spread of Mycobacterium avium subsp. paratuberculosis Through Soil and Grass on a Mouflon (Ovis aries) Pasture

  • Marija Kaevska
  • S. Lvoncik
  • J. Lamka
  • I. Pavlik
  • I. Slana


The aims of this study were to describe spatial contamination of the environment on a mouflon pasture, as well as to assess the contamination of grass and roots after surface contamination and in depth contamination with feces and buried tissues from animals infected with Mycobacterium avium subsp. paratuberculosis (M. a. paratuberculosis). Samples of soil, roots, and aerial parts of plants were collected from different locations inside the mouflon pasture, and one control sample site was chosen outside the area where the animals are living. M. a. paratuberculosis DNA was present in all the examined sites and was more often detected in roots than in soil. DNA was detected at up to 80 cm of depth and was spatially more widespread than the initial hypothesis of M. a. paratuberculosis leaching vertically into deeper layers of soil. This study broadens our knowledge of the spread and persistence of M. a. paratuberculosis in an environment with highly infected animals.



The work was supported by Grant from the Ministry of Agriculture (No. MZe0002716202). The results of the project LO1218 were obtained with a financial support from the Ministry of Education, Youth and Sports (MEYS) of the Czech Republic under the NPU I program.

Conflict of interest

The authors declare that there is no conflict of interests regarding the publication of this article.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Marija Kaevska
    • 1
  • S. Lvoncik
    • 2
  • J. Lamka
    • 3
  • I. Pavlik
    • 1
  • I. Slana
    • 1
  1. 1.Veterinary Research InstituteBrnoCzech Republic
  2. 2.Department of Plant BiologyMendel UniversityBrnoCzech Republic
  3. 3.Faculty of Pharmacy in Hradec KraloveCharles University in PraguePragueCzech Republic

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