Advertisement

Current Microbiology

, Volume 69, Issue 3, pp 245–251 | Cite as

Helicobacter pylori Identification: A Diagnostic/Confirmatory Method for Evaluation

  • B. Mesquita
  • M. J. Gonçalves
  • P. Pacheco
  • J. Lopes
  • F. Salazar
  • M. Relvas
  • C. Coelho
  • J. J. Pacheco
  • C. VelazcoEmail author
Article

Abstract

The Helicobacter pylori extra gastric reservoir is probably the oral cavity. In order to evaluate the presence of this bacterium in patients with periodontitis and suspicious microbial cultures, saliva was collected from these and non-periodontitis subjects. PCRs targeting 16S rRNA gene and a 860 bp specific region were performed, and digested with the restriction enzyme DdeI. We observed that the PCR–RFLP approach augments the accuracy from 26.2 % (16/61), found in the PCR-based results, to 42.6 % (26/61), which is an excellent indicator for the establishment of this low-cost procedure as a diagnostic/confirmatory method for H. pylori evaluation.

Keywords

Polymerase Chain Reaction Periodontitis Saliva Sample Nest Polymerase Chain Reaction Dental Plaque 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was supported by 03—IINFACTS/CESPU, 2011. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

References

  1. 1.
    Bernander S, Dalen J, Gastrin B, Hedenborg L, Lamke LO, Ohrn R (1993) Absence of Helicobacter pylori in dental plaques in Helicobacter pylori positive dyspeptic patients. Eur J Clin Microbiol Infect Dis 12(4):282–285PubMedCrossRefGoogle Scholar
  2. 2.
    Brown LM (2000) Helicobacter pylori: epidemiology and routes of transmission. Epidemiol Rev 22(2):283–297PubMedCrossRefGoogle Scholar
  3. 3.
    Chaudhry S, Idrees M, Izhar M, Butt AK, Khan AA (2011) Simultaneous amplification of two bacterial genes: more reliable method of Helicobacter pylori detection in microbial rich dental plaque samples. Curr Microbiol 62(1):78–83. doi: 10.1007/s00284-010-9662-x PubMedCrossRefGoogle Scholar
  4. 4.
    Chaudhry S, Iqbal HA, Khan AA, Izhar M, Butt AK, Akhter MW, Izhar F, Mirza KM (2008) Helicobacter pylori in dental plaque and gastric mucosa: correlation revisited. J Pak Med Assoc 58(6):331–334PubMedGoogle Scholar
  5. 5.
    Cheng LH, Webberley M, Evans M, Hanson N, Brown R (1996) Helicobacter pylori in dental plaque and gastric mucosa. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 81(4):421–423PubMedCrossRefGoogle Scholar
  6. 6.
    Feldman RA, Eccersley AJ, Hardie JM (1998) Epidemiology of Helicobacter pylori: acquisition, transmission, population prevalence and disease-to-infection ratio. Br Med Bull 54(1):39–53PubMedCrossRefGoogle Scholar
  7. 7.
    Gonzalez A, Moreno Y, Gonzalez R, Hernandez J, Ferrus MA (2006) Development of a simple and rapid method based on polymerase chain reaction-based restriction fragment length polymorphism analysis to differentiate Helicobacter, Campylobacter, and Arcobacter species. Curr Microbiol 53(5):416–421. doi: 10.1007/s00284-006-0168-5 PubMedCrossRefGoogle Scholar
  8. 8.
    Ishihara K, Miura T, Ebihara Y, Hirayama T, Kamiya S, Okuda K (2001) Shared antigenicity between Helicobacter pylori and periodontopathic Campylobacter rectus strains. FEMS Microbiol Lett 197(1):23–27PubMedCrossRefGoogle Scholar
  9. 9.
    Kabir S (2004) Detection of Helicobacter pylori DNA in feces and saliva by polymerase chain reaction: a review. Helicobacter 9(2):115–123. doi: 10.1111/j.1083-4389.2004.00207.x PubMedCrossRefGoogle Scholar
  10. 10.
    Li C, Ha T, Ferguson DA Jr, Chi DS, Zhao R, Patel NR, Krishnaswamy G, Thomas E (1996) A newly developed PCR assay of H. pylori in gastric biopsy, saliva, and feces. Evidence of high prevalence of H. pylori in saliva supports oral transmission. Dig Dis Sci 41(11):2142–2149PubMedCrossRefGoogle Scholar
  11. 11.
    Li C, Musich PR, Ha T, Ferguson DA Jr, Patel NR, Chi DS, Thomas E (1995) High prevalence of Helicobacter pylori in saliva demonstrated by a novel PCR assay. J Clin Pathol 48(7):662–666PubMedCentralPubMedCrossRefGoogle Scholar
  12. 12.
    Loster BW, Majewski SW, Czesnikiewicz-Guzik M, Bielanski W, Pierzchalski P, Konturek SJ (2006) The relationship between the presence of Helicobacter pylori in the oral cavity and gastric in the stomach. J Physiol Pharmacol 57(Suppl 3):91–100PubMedGoogle Scholar
  13. 13.
    Luman W, Alkout AM, Blackwell CC, Weir DM, Plamer KR (1996) Helicobacter pylori in the mouth-negative isolation from dental plaque and saliva. Eur J Gastroenterol Hepatol 8(1):11–14PubMedCrossRefGoogle Scholar
  14. 14.
    Mapstone NP, Lynch DA, Lewis FA, Axon AT, Tompkins DS, Dixon MF, Quirke P (1993) Identification of Helicobacter pylori DNA in the mouths and stomachs of patients with gastritis using PCR. J Clin Pathol 46(6):540–543PubMedCentralPubMedCrossRefGoogle Scholar
  15. 15.
    Mbulaiteye SM, Hisada M, El-Omar EM (2009) Helicobacter pylori associated global gastric cancer burden. Front Biosci (Landmark Ed) 14:1490–1504CrossRefGoogle Scholar
  16. 16.
    Medina ML, Medina MG, Martin GT, Picon SO, Bancalari A, Merino LA (2010) Molecular detection of Helicobacter pylori in oral samples from patients suffering digestive pathologies. Med Oral Patol Oral Cir Bucal 15(1):e38–e42PubMedGoogle Scholar
  17. 17.
    Momtaz H, Souod N, Dabiri H, Sarshar M (2012) Study of Helicobacter pylori genotype status in saliva, dental plaques, stool and gastric biopsy samples. World J Gastroenterol 18(17):2105–2111. doi: 10.3748/wjg.v18.i17.2105 PubMedCentralPubMedCrossRefGoogle Scholar
  18. 18.
    Olivier BJ, Bond RP, van Zyl WB, Delport M, Slavik T, Ziady C, Terhaar Sive Droste JS, Lastovica A, van der Merwe SW (2006) Absence of Helicobacter pylori within the oral cavities of members of a healthy South African community. J Clin Microbiol 44(2):635–636. doi: 10.1128/JCM.44.2.635-636.2006 PubMedCentralPubMedCrossRefGoogle Scholar
  19. 19.
    Osaki T, Mabe K, Hanawa T, Kamiya S (2008) Urease-positive bacteria in the stomach induce a false-positive reaction in a urea breath test for diagnosis of Helicobacter pylori infection. J Med Microbiol 57(Pt 7):814–819. doi: 10.1099/jmm.0.47768-0 PubMedCrossRefGoogle Scholar
  20. 20.
    Pacifico L, Anania C, Osborn JF, Ferraro F, Chiesa C (2010) Consequences of Helicobacter pylori infection in children. World J Gastroenterol 16(41):5181–5194PubMedCentralPubMedCrossRefGoogle Scholar
  21. 21.
    Parsonnet J, Shmuely H, Haggerty T (1999) Fecal and oral shedding of Helicobacter pylori from healthy infected adults. J Am Med Assoc 282(23):2240–2245CrossRefGoogle Scholar
  22. 22.
    Salih BA (2009) Helicobacter pylori infection in developing countries: the burden for how long? Saudi J Gastroenterol 15(3):201–207. doi: 10.4103/1319-3767.54743 PubMedCentralPubMedCrossRefGoogle Scholar
  23. 23.
    Silva DG, Tinoco EM, Rocha GA, Rocha AM, Guerra JB, Saraiva IE, Queiroz DM (2010) Helicobacter pylori transiently in the mouth may participate in the transmission of infection. Mem Inst Oswaldo Cruz 105(5):657–660PubMedCrossRefGoogle Scholar
  24. 24.
    Song Q, Haller B, Ulrich D, Wichelhaus A, Adler G, Bode G (2000) Quantitation of Helicobacter pylori in dental plaque samples by competitive polymerase chain reaction. J Clin Pathol 53(3):218–222PubMedCentralPubMedCrossRefGoogle Scholar
  25. 25.
    Sugimoto M, Wu JY, Abudayyeh S, Hoffman J, Brahem H, Al-Khatib K, Yamaoka Y, Graham DY (2009) Unreliability of results of PCR detection of Helicobacter pylori in clinical or environmental samples. J Clin Microbiol 47(3):738–742. doi: 10.1128/JCM.01563-08 PubMedCentralPubMedCrossRefGoogle Scholar
  26. 26.
    Suzuki R, Shiota S, Yamaoka Y (2012) Molecular epidemiology, population genetics, and pathogenic role of Helicobacter pylori. Infect Genet Evol 12(2):203–213. doi: 10.1016/j.meegid.2011.12.002 PubMedCentralPubMedCrossRefGoogle Scholar
  27. 27.
    Talley NJ, Li Z (2007) Helicobacter pylori: testing and treatment. Expert Rev Gastroenterol Hepatol 1(1):71–79. doi: 10.1586/17474124.1.1.71 PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • B. Mesquita
    • 1
  • M. J. Gonçalves
    • 1
  • P. Pacheco
    • 1
  • J. Lopes
    • 1
  • F. Salazar
    • 1
  • M. Relvas
    • 1
  • C. Coelho
    • 1
  • J. J. Pacheco
    • 1
  • C. Velazco
    • 2
    Email author
  1. 1.Instituto de Investigação e Formação Avançada em Técnicas da Saúde (IINFACTS)Instituto Superior de Ciências da Saúde-Norte/CESPUGandra PRDPortugal
  2. 2.CESPU–ISCSNGandra PRDPortugal

Personalised recommendations