Current Microbiology

, Volume 69, Issue 3, pp 245–251 | Cite as

Helicobacter pylori Identification: A Diagnostic/Confirmatory Method for Evaluation

  • B. Mesquita
  • M. J. Gonçalves
  • P. Pacheco
  • J. Lopes
  • F. Salazar
  • M. Relvas
  • C. Coelho
  • J. J. Pacheco
  • C. VelazcoEmail author


The Helicobacter pylori extra gastric reservoir is probably the oral cavity. In order to evaluate the presence of this bacterium in patients with periodontitis and suspicious microbial cultures, saliva was collected from these and non-periodontitis subjects. PCRs targeting 16S rRNA gene and a 860 bp specific region were performed, and digested with the restriction enzyme DdeI. We observed that the PCR–RFLP approach augments the accuracy from 26.2 % (16/61), found in the PCR-based results, to 42.6 % (26/61), which is an excellent indicator for the establishment of this low-cost procedure as a diagnostic/confirmatory method for H. pylori evaluation.


Polymerase Chain Reaction Periodontitis Saliva Sample Nest Polymerase Chain Reaction Dental Plaque 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This study was supported by 03—IINFACTS/CESPU, 2011. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • B. Mesquita
    • 1
  • M. J. Gonçalves
    • 1
  • P. Pacheco
    • 1
  • J. Lopes
    • 1
  • F. Salazar
    • 1
  • M. Relvas
    • 1
  • C. Coelho
    • 1
  • J. J. Pacheco
    • 1
  • C. Velazco
    • 2
    Email author
  1. 1.Instituto de Investigação e Formação Avançada em Técnicas da Saúde (IINFACTS)Instituto Superior de Ciências da Saúde-Norte/CESPUGandra PRDPortugal
  2. 2.CESPU–ISCSNGandra PRDPortugal

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