Current Microbiology

, Volume 68, Issue 5, pp 621–628 | Cite as

Role of the Flagellar Basal-Body Protein, FlgC, in the Binding of Salmonella enterica Serovar Enteritidis to Host Cells

  • Daniel C. Shippy
  • Nicholas M. Eakley
  • Dareen M. Mikheil
  • Amin A. Fadl
Article
First Online:
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Accepted:

Abstract

Salmonella enterica serovar Enteritidis (SE) infection in humans is often associated with the consumption of contaminated poultry products. Binding of the bacterium to the intestinal mucosa is a major pathogenic mechanism of Salmonella in poultry. Transposon mutagenesis identified flgC as a potential binding mutant of SE. Therefore, we hypothesize FlgC which plays a significant role in the binding ability of SE to the intestinal mucosa of poultry. To test our hypothesis, we created a mutant of SE in which flgC was deleted. We then tested the in vitro and in vivo binding ability of ∆flgC when compared to the wild-type SE strain. Our data showed a significant decrease in the binding ability of ∆flgC to intestinal epithelial cells as well as in the small intestine and cecum of poultry. Furthermore, the decrease in binding correlated to a defect in invasion as shown by a cell culture model using intestinal epithelial cells and bacterial recovery from the livers and spleens of chickens. Overall, these studies indicate FlgC is a major factor in the binding ability of Salmonella to the intestinal mucosa of poultry.

Keywords

FlgC Binding Salmonella Poultry 
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Notes

Acknowledgments

We thank Dr. Charles Kaspar, University of Wisconsin-Madison, for providing the WT SE PT8 isolate. This work was supported by a grant from the University of Wisconsin-Madison Graduate School.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Daniel C. Shippy
    • 1
  • Nicholas M. Eakley
    • 1
  • Dareen M. Mikheil
    • 1
  • Amin A. Fadl
    • 1
  1. 1.Department of Animal SciencesUniversity of Wisconsin-MadisonMadisonUSA

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