Current Microbiology

, Volume 68, Issue 4, pp 536–542 | Cite as

Development of SCAR Markers to Determine the Mating Types of Lepista nuda Protoplast Monokaryons

  • Dengjin Li
  • Yu Liu
  • Peng Wang
  • Yuanwei Ma
  • Shouxian Wang
  • Shuang Zhao
  • Feng Xu
Article
First Online:
Received:
Accepted:

Abstract

Lepista nuda (Bull. ex Fr.) Cooke belongs to Tricholomataceae and is an edible fungus with both economic and medical value. Mycelia were isolated from the fruiting bodies of L. nuda and were used to prepare the protoplast monokaryons. One hundred and fifteen monokaryons were obtained and their mating types were determined using somatic incompatibility tests. Protoplast monokaryons segregated into either the A1B1 or the A2B2 mating types. Inter-simple sequence repeats and sequence-related amplified polymorphism fingerprinting were used to analyse the mating types of these protoplast monokaryons and 16 sequence-characterised amplified region primers were developed to efficiently differentiate between the monokaryon mating types. Multiplex PCR analyses were also established. The data presented here outline a method for the precise and rapid identification of protoplast monokaryon mating types, which has the promise to shorten the period required for conventional crossbreeding.

Keywords

Mating Type Scar Marker Clamp Connection Ethidium Bromide Solution Pleurotus Eryngii 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

Thanks to the anonymous reviewers for improvement on this paper. Thanks to Prof. Xinghong Li and Dr. Jiye Yan for providing Lepista nuda strain. This work was supported by Grants from the Beijing Nova Program (Grant No. 2011053) and Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Science (Grant No. CCJJZDXM201201-3)

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

284_2013_510_MOESM1_ESM.doc (500 kb)
Supplementary material 1 (DOC 500 kb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Dengjin Li
    • 1
    • 2
  • Yu Liu
    • 1
  • Peng Wang
    • 1
  • Yuanwei Ma
    • 1
  • Shouxian Wang
    • 1
  • Shuang Zhao
    • 1
  • Feng Xu
    • 1
  1. 1.Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry SciencesBeijing Engineering Research Center for Edible Mushroom, Key Laboratory of Urban Agriculture (North), Ministry of AgricultureBeijingChina
  2. 2.University of Chinese Academy of ScienceBeijingChina

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