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Current Microbiology

, Volume 68, Issue 4, pp 419–427 | Cite as

Stability of the Maternal Gut Microbiota During Late Pregnancy and Early Lactation

  • Ted Jost
  • Christophe Lacroix
  • Christian Braegger
  • Christophe Chassard
Article

Abstract

Scarce research has been performed to assess whether the human maternal gut microbiota undergoes changes during the perinatal period. Therefore, in the present study, gut microbiota composition of seven healthy mothers(to-be) was assessed at different time points during the perinatal period (i.e. weeks 3–7 prepartum and days 3–6, 9–14, and 25–30 postpartum) using quantitative polymerase chain reaction (qPCR) and pyrosequencing, and was complemented by short-chain fatty acids (SCFA) and calprotectin quantification using high-performance liquid chromatography and enzyme-linked immunosorbent assay, respectively. qPCR revealed the predominance of members of the Firmicutes, Bacteroides, and Bifidobacterium without detectable changes over the perinatal period. Pyrosequencing supported these data in terms of microbiota stability for any population at any taxonomic level, although ratios of members of the Actinobacteria and Bacteroidetes differed between the two methods. However, the number of operational taxonomic units observed by pyrosequencing was subjected to fluctuations and the relative abundance of Streptococcus decreased numerically postpartum (P = 0.11), which may indicate that aberrancies in subdominant populations occur perinatally. Furthermore, total fecal SCFA concentrations, particularly the branched-chain fatty acids isobutyrate and isovalerate, were higher than for non-pregnant subjects throughout the perinatal period. This suggests metabolic changes and increased energy extraction via proteolytic, in addition to saccharolytic fermentation, accompanied by low-grade inflammation based on fecal calprotectin levels. Our data show that the maternal gut microbiota remained stable over the perinatal period despite altered metabolic activity and low-grade inflammation; however, it remains to be confirmed whether changes preceded earlier during pregnancy and succeeded later postpartum.

Keywords

Actinobacteria Firmicutes Bacteroidetes Perinatal Period Total Bacterium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work has been supported by Nestlé Nutrition (Vevey, Switzerland), Nestec (Lausanne, Switzerland), and the Swiss Foundation for Nutrition Research (SFEFS) (Zurich, Switzerland) for the purpose of basic research only. The authors are grateful to Patrick Bühr, Michael Friedt, Petra Martel, Daniela Rogler, and Rebekka Koller at the University Children’s Hospital Zurich for their effort in volunteer recruitment and sampling, and to Valérie Béguin for assisting in qPCR analyses, carried out at the Genetic Diversity Centre of ETH Zurich (Zurich, Switzerland).

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Ted Jost
    • 1
  • Christophe Lacroix
    • 1
  • Christian Braegger
    • 2
  • Christophe Chassard
    • 1
  1. 1.Laboratory of Food Biotechnology, Institute of Food, Nutrition and HealthETH ZurichZurichSwitzerland
  2. 2.Division of Gastroenterology and NutritionUniversity Children’s Hospital ZurichZurichSwitzerland

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