Current Microbiology

, Volume 68, Issue 2, pp 227–232 | Cite as

pIMP-PH114 Carrying bla IMP-4 in a Klebsiella pneumoniae Strain is Closely Related to Other Multidrug-Resistant IncA/C2 Plasmids

  • Pak-Leung HoEmail author
  • Wai-U Lo
  • Jane Chan
  • Yuk-Yam Cheung
  • Kin-Hung Chow
  • Wing-Cheong Yam
  • Chi-Ho Lin
  • Tak-Lun Que


The IncA/C plasmids are broad host-range vehicles which have been associated with wide dissemination of CMY-2 among Enterobacteriaceae of human and animal origins. Acquired metallo-β-lactamases (MBLs) such as the IMP-type enzymes are increasingly reported in multidrug-resistant Gram-negative bacteria worldwide, particularly in Enterobacteriaceae. We described the complete sequence of the first IMP-4-encoding IncA/C2 plasmid, pIMP-PH114 (151,885 bp), from a sequence type 1 Klebsiella pneumoniae strain that was recovered from a patient who was hospitalized in the Philippines. pIMP-PH114 consists of a backbone from the IncA/C2 plasmids, with the insertion of a novel Tn21-like class 1 integron composite structure (containing the cassette array bla IMP-4-qacG-aacA4-catB3, followed by a class C β-lactamase bla DHA-1 and the mercury resistance operon, merRTPCADE) and a sul2-floR encoding region. Phylogenetic analysis of the IncA/C repA sequences showed that pIMP-PH114 formed a subgroup with other IncA/C plasmids involved in the international spread of CMY-2, TEM-24 and NDM-1. Identical bla IMP-4 arrays have been described among different Enterobacteriaceae and Acinetobacter spp. in China, Singapore and Australia but the genetic context is different. The broad host range of IncA/C plasmids may have facilitated dissemination of the bla IMP-4 arrays among different diverse groups of bacteria.


Tigecycline Boronic Acid Ertapenem Incompatibility Group Florfenicol 
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This work was supported by grants from the Research Fund for the Control of Infectious Diseases (RFCID) of the Health and Food Bureau of the Government of the HKSAR. We thank Pak-Chung Sham, Levina Lam, Ben Ho and Wilson Chan of the Centre for Genomic Science, University of Hong Kong for technical assistance. Part of the data was presented as an abstract at the 23rd European Society of Clinical Microbiology and Infectious Diseases (ECCMID) meeting, Berlin, Germany, 27–30 April 2013.

Supplementary material

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Supplementary material 1 (DOCX 97 kb)


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Pak-Leung Ho
    • 1
    Email author
  • Wai-U Lo
    • 1
  • Jane Chan
    • 1
  • Yuk-Yam Cheung
    • 1
  • Kin-Hung Chow
    • 1
  • Wing-Cheong Yam
    • 1
  • Chi-Ho Lin
    • 2
  • Tak-Lun Que
    • 3
  1. 1.Department of MicrobiologyQueen Mary hospital and Carol Yu Centre for Infection, The University of Hong KongPokfulamChina
  2. 2.Centre for Genomic SciencesThe University of Hong KongPokfulamChina
  3. 3.Department of Clinical PathologyTuen Mun Hospital, Hospital AuthorityPokfulamChina

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