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Current Microbiology

, Volume 68, Issue 2, pp 227–232 | Cite as

pIMP-PH114 Carrying bla IMP-4 in a Klebsiella pneumoniae Strain is Closely Related to Other Multidrug-Resistant IncA/C2 Plasmids

  • Pak-Leung HoEmail author
  • Wai-U Lo
  • Jane Chan
  • Yuk-Yam Cheung
  • Kin-Hung Chow
  • Wing-Cheong Yam
  • Chi-Ho Lin
  • Tak-Lun Que
Article

Abstract

The IncA/C plasmids are broad host-range vehicles which have been associated with wide dissemination of CMY-2 among Enterobacteriaceae of human and animal origins. Acquired metallo-β-lactamases (MBLs) such as the IMP-type enzymes are increasingly reported in multidrug-resistant Gram-negative bacteria worldwide, particularly in Enterobacteriaceae. We described the complete sequence of the first IMP-4-encoding IncA/C2 plasmid, pIMP-PH114 (151,885 bp), from a sequence type 1 Klebsiella pneumoniae strain that was recovered from a patient who was hospitalized in the Philippines. pIMP-PH114 consists of a backbone from the IncA/C2 plasmids, with the insertion of a novel Tn21-like class 1 integron composite structure (containing the cassette array bla IMP-4-qacG-aacA4-catB3, followed by a class C β-lactamase bla DHA-1 and the mercury resistance operon, merRTPCADE) and a sul2-floR encoding region. Phylogenetic analysis of the IncA/C repA sequences showed that pIMP-PH114 formed a subgroup with other IncA/C plasmids involved in the international spread of CMY-2, TEM-24 and NDM-1. Identical bla IMP-4 arrays have been described among different Enterobacteriaceae and Acinetobacter spp. in China, Singapore and Australia but the genetic context is different. The broad host range of IncA/C plasmids may have facilitated dissemination of the bla IMP-4 arrays among different diverse groups of bacteria.

Keywords

Tigecycline Boronic Acid Ertapenem Incompatibility Group Florfenicol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by grants from the Research Fund for the Control of Infectious Diseases (RFCID) of the Health and Food Bureau of the Government of the HKSAR. We thank Pak-Chung Sham, Levina Lam, Ben Ho and Wilson Chan of the Centre for Genomic Science, University of Hong Kong for technical assistance. Part of the data was presented as an abstract at the 23rd European Society of Clinical Microbiology and Infectious Diseases (ECCMID) meeting, Berlin, Germany, 27–30 April 2013.

Supplementary material

284_2013_471_MOESM1_ESM.docx (98 kb)
Supplementary material 1 (DOCX 97 kb)

References

  1. 1.
    Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M et al (2008) The RAST Server: rapid annotations using subsystems technology. BMC Genomics 9:75PubMedCentralPubMedCrossRefGoogle Scholar
  2. 2.
    Carattoli A (2013) Plasmids and the spread of resistance. Int J Med Microbiol 303(6–7):298–304PubMedCrossRefGoogle Scholar
  3. 3.
    Carattoli A, Villa L, Poirel L, Bonnin RA, Nordmann P (2012) Evolution of IncA/C blaCMY-(2)-carrying plasmids by acquisition of the blaNDM-(1) carbapenemase gene. Antimicrob Agents Chemother 56:783–786PubMedCentralPubMedCrossRefGoogle Scholar
  4. 4.
    Clinical and Laboratory Standards Institute (2012) Performance standards for antimicrobial susceptibility testing: twenty-second informational supplement M100-S22. CLSI, Wayne, PA, USAGoogle Scholar
  5. 5.
    Cornaglia G, Giamarellou H, Rossolini GM (2011) Metallo-beta-lactamases: a last frontier for beta-lactams? Lancet Infect Dis 11:381–393PubMedCrossRefGoogle Scholar
  6. 6.
    Espedido BA, Partridge SR, Iredell JR (2008) bla(IMP-4) in different genetic contexts in Enterobacteriaceae isolates from Australia. Antimicrob Agents Chemother 52:2984–2987PubMedCentralPubMedCrossRefGoogle Scholar
  7. 7.
    Fernandez-Alarcon C, Singer RS, Johnson TJ (2011) Comparative genomics of multidrug resistance-encoding IncA/C plasmids from commensal and pathogenic Escherichia coli from multiple animal sources. PLoS ONE 6:e23415PubMedCentralPubMedCrossRefGoogle Scholar
  8. 8.
    Fricke WF, Welch TJ, McDermott PF, Mammel MK, LeClerc JE, White DG, Cebula TA, Ravel J (2009) Comparative genomics of the IncA/C multidrug resistance plasmid family. J Bacteriol 191:4750–4757PubMedCentralPubMedCrossRefGoogle Scholar
  9. 9.
    Hanau-Bercot B, Podglajen I, Casin I, Collatz E (2002) An intrinsic control element for translational initiation in class 1 integrons. Mol Microbiol 44:119–130PubMedCrossRefGoogle Scholar
  10. 10.
    Ho PL, Li Z, Lo WU, Cheung YY, Lin CH, Sham PC, Cheng VC, Ng TK, Chow KH (2012) Identification and characterization of a novel incompatibility group X3 plasmid carrying bla NDM-1 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China. Emerg Microbes Infect 1:e39PubMedCentralCrossRefGoogle Scholar
  11. 11.
    Ho PL, Lo WU, Yeung MK, Lin CH, Chow KH, Ang I, Tong AH, Bao JY, Lok S, Lo JY (2011) Complete sequencing of pNDM-HK encoding NDM-1 carbapenemase from a multidrug-resistant Escherichia coli strain isolated in Hong Kong. PLoS ONE 6:e17989PubMedCentralPubMedCrossRefGoogle Scholar
  12. 12.
    Houang ET, Chu YW, Lo WS, Chu KY, Cheng AF (2003) Epidemiology of rifampin ADP-ribosyltransferase (arr-2) and metallo-beta-lactamase (blaIMP-4) gene cassettes in class 1 integrons in Acinetobacter strains isolated from blood cultures in 1997–2000. Antimicrob Agents Chemother 47:1382–1390PubMedCentralPubMedCrossRefGoogle Scholar
  13. 13.
    Johnson TJ, Bielak EM, Fortini D, Hansen LH, Hasman H, Debroy C, Nolan LK, Carattoli A (2012) Expansion of the IncX plasmid family for improved identification and typing of novel plasmids in drug-resistant Enterobacteriaceae. Plasmid 68:43–50PubMedCrossRefGoogle Scholar
  14. 14.
    Johnson TJ, Lang KS (2012) IncA/C plasmids: an emerging threat to human and animal health? Mob Genet Elements 2:55–58PubMedCentralPubMedCrossRefGoogle Scholar
  15. 15.
    Johnson TJ, Nolan LK (2009) Plasmid replicon typing. Methods Mol Biol 551:27–35PubMedCrossRefGoogle Scholar
  16. 16.
    Koh TH, Sng LH, Wang GC, Hsu LY, Zhao Y (2007) IMP-4 and OXA beta-lactamases in Acinetobacter baumannii from Singapore. J Antimicrob Chemother 59:627–632PubMedCrossRefGoogle Scholar
  17. 17.
    Lo WU, Cheung YY, Lai E, Lung D, Que TL, Ho PL (2013) Complete sequence of an IncN plasmid, pIMP-HZ1, carrying blaIMP-4 in a Klebsiella pneumoniae strain associated with medical travel to China. Antimicrob Agents Chemother 57:1561–1562PubMedCentralPubMedCrossRefGoogle Scholar
  18. 18.
    Moura A, Soares M, Pereira C, Leitao N, Henriques I, Correia A (2009) INTEGRALL: a database and search engine for integrons, integrases and gene cassettes. Bioinformatics 25:1096–1098PubMedCrossRefGoogle Scholar
  19. 19.
    Novais A, Baquero F, Machado E, Canton R, Peixe L, Coque TM (2010) International spread and persistence of TEM-24 is caused by the confluence of highly penetrating Enterobacteriaceae clones and an IncA/C2 plasmid containing Tn1696:Tn1 and IS5075-Tn21. Antimicrob Agents Chemother 54:825–834PubMedCentralPubMedCrossRefGoogle Scholar
  20. 20.
    Partridge SR, Ginn AN, Paulsen IT, Iredell JR (2012) pEl1573 Carrying blaIMP-4, from Sydney, Australia, is closely related to other IncL/M plasmids. Antimicrob Agents Chemother 56:6029–6032PubMedCentralPubMedCrossRefGoogle Scholar
  21. 21.
    Partridge SR, Hall RM (2003) The IS1111 family members IS4321 and IS5075 have subterminal inverted repeats and target the terminal inverted repeats of Tn21 family transposons. J Bacteriol 185:6371–6384PubMedCentralPubMedCrossRefGoogle Scholar
  22. 22.
    Partridge SR, Paulsen IT, Iredell JR (2012) pJIE137 carrying blaCTX-M-62 is closely related to p271A carrying blaNDM-1. Antimicrob Agents Chemother 56:2166–2168PubMedCentralPubMedCrossRefGoogle Scholar
  23. 23.
    Partridge SR, Tsafnat G, Coiera E, Iredell JR (2009) Gene cassettes and cassette arrays in mobile resistance integrons. FEMS Microbiol Rev 33:757–784PubMedCrossRefGoogle Scholar
  24. 24.
    Sekizuka T, Matsui M, Yamane K, Takeuchi F, Ohnishi M, Hishinuma A, Arakawa Y, Kuroda M (2011) Complete sequencing of the bla(NDM-1)-positive IncA/C plasmid from Escherichia coli ST38 isolate suggests a possible origin from plant pathogens. PLoS ONE 6:e25334PubMedCentralPubMedCrossRefGoogle Scholar
  25. 25.
    Zaidi MB, Leon V, Canche C, Perez C, Zhao S, Hubert SK, Abbott J, Blickenstaff K, McDermott PF (2007) Rapid and widespread dissemination of multidrug-resistant blaCMY-2 Salmonella typhimurium in Mexico. J Antimicrob Chemother 60:398–401PubMedCrossRefGoogle Scholar
  26. 26.
    Zhao WH, Hu ZQ (2011) IMP-type metallo-beta-lactamases in Gram-negative bacilli: distribution, phylogeny, and association with integrons. Crit Rev Microbiol 37:214–226PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Pak-Leung Ho
    • 1
    Email author
  • Wai-U Lo
    • 1
  • Jane Chan
    • 1
  • Yuk-Yam Cheung
    • 1
  • Kin-Hung Chow
    • 1
  • Wing-Cheong Yam
    • 1
  • Chi-Ho Lin
    • 2
  • Tak-Lun Que
    • 3
  1. 1.Department of MicrobiologyQueen Mary hospital and Carol Yu Centre for Infection, The University of Hong KongPokfulamChina
  2. 2.Centre for Genomic SciencesThe University of Hong KongPokfulamChina
  3. 3.Department of Clinical PathologyTuen Mun Hospital, Hospital AuthorityPokfulamChina

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