pIMP-PH114 Carrying bla IMP-4 in a Klebsiella pneumoniae Strain is Closely Related to Other Multidrug-Resistant IncA/C2 Plasmids
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The IncA/C plasmids are broad host-range vehicles which have been associated with wide dissemination of CMY-2 among Enterobacteriaceae of human and animal origins. Acquired metallo-β-lactamases (MBLs) such as the IMP-type enzymes are increasingly reported in multidrug-resistant Gram-negative bacteria worldwide, particularly in Enterobacteriaceae. We described the complete sequence of the first IMP-4-encoding IncA/C2 plasmid, pIMP-PH114 (151,885 bp), from a sequence type 1 Klebsiella pneumoniae strain that was recovered from a patient who was hospitalized in the Philippines. pIMP-PH114 consists of a backbone from the IncA/C2 plasmids, with the insertion of a novel Tn21-like class 1 integron composite structure (containing the cassette array bla IMP-4-qacG-aacA4-catB3, followed by a class C β-lactamase bla DHA-1 and the mercury resistance operon, merRTPCADE) and a sul2-floR encoding region. Phylogenetic analysis of the IncA/C repA sequences showed that pIMP-PH114 formed a subgroup with other IncA/C plasmids involved in the international spread of CMY-2, TEM-24 and NDM-1. Identical bla IMP-4 arrays have been described among different Enterobacteriaceae and Acinetobacter spp. in China, Singapore and Australia but the genetic context is different. The broad host range of IncA/C plasmids may have facilitated dissemination of the bla IMP-4 arrays among different diverse groups of bacteria.
KeywordsTigecycline Boronic Acid Ertapenem Incompatibility Group Florfenicol
This work was supported by grants from the Research Fund for the Control of Infectious Diseases (RFCID) of the Health and Food Bureau of the Government of the HKSAR. We thank Pak-Chung Sham, Levina Lam, Ben Ho and Wilson Chan of the Centre for Genomic Science, University of Hong Kong for technical assistance. Part of the data was presented as an abstract at the 23rd European Society of Clinical Microbiology and Infectious Diseases (ECCMID) meeting, Berlin, Germany, 27–30 April 2013.
- 4.Clinical and Laboratory Standards Institute (2012) Performance standards for antimicrobial susceptibility testing: twenty-second informational supplement M100-S22. CLSI, Wayne, PA, USAGoogle Scholar
- 10.Ho PL, Li Z, Lo WU, Cheung YY, Lin CH, Sham PC, Cheng VC, Ng TK, Chow KH (2012) Identification and characterization of a novel incompatibility group X3 plasmid carrying bla NDM-1 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China. Emerg Microbes Infect 1:e39PubMedCentralCrossRefGoogle Scholar
- 12.Houang ET, Chu YW, Lo WS, Chu KY, Cheng AF (2003) Epidemiology of rifampin ADP-ribosyltransferase (arr-2) and metallo-beta-lactamase (blaIMP-4) gene cassettes in class 1 integrons in Acinetobacter strains isolated from blood cultures in 1997–2000. Antimicrob Agents Chemother 47:1382–1390PubMedCentralPubMedCrossRefGoogle Scholar
- 19.Novais A, Baquero F, Machado E, Canton R, Peixe L, Coque TM (2010) International spread and persistence of TEM-24 is caused by the confluence of highly penetrating Enterobacteriaceae clones and an IncA/C2 plasmid containing Tn1696:Tn1 and IS5075-Tn21. Antimicrob Agents Chemother 54:825–834PubMedCentralPubMedCrossRefGoogle Scholar