Current Microbiology

, Volume 68, Issue 1, pp 105–112 | Cite as

gltB/D Mutants of Xanthomonas oryzae pv. oryzae are Virulence Deficient

  • Alok Pandey
  • Suvendra Kumar Ray
  • Ramesh V. Sonti
  • R. Rajeshwari
Article
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Abstract

Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight, a serious disease of rice. Upon clip inoculation of rice leaves, Xoo causes typical V-shaped lesions whose leading edge moves through the mid-veinal region. We have isolated a virulence deficient mutant of Xoo, referred to as BXO808 that causes limited lesions which primarily extend through the side-veinal regions of rice leaves. Functional complementation studies identified a clone, pSR19, from a cosmid genomic library that restored wild-type virulence and lesion phenotype to BXO808. Transposon mutagenesis of the pSR19 clone, marker exchange experiments, and targeted mutagenesis, revealed that the BXO808 phenotype is due to mutation in the gltB/D genes of Xoo, which encode glutamate synthase subunits α and β, respectively. The gltB/D mutants that were generated in this study also exhibited virulence deficiency, an altered lesion phenotype and growth deficiency on minimal medium with low levels of ammonium as a sole nitrogen source. This is the first report that mutations in the gltB/D genes of Xoo cause virulence deficiency.

Keywords

Rice Leave Virulence Deficiency Marker Exchange Minimal Glucose Medium Diffusible Signaling Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

Alok Pandey was supported by research fellowships from the Council of Scientific and Industrial Research (CSIR) and Department of Biotechnology, Government of India. Suvendra Kumar Ray was supported by a senior research fellowship from CSIR, Government of India. We gratefully acknowledge the help of G. Jyotsna in the sequencing of pSR19 clone. This work was supported, in part, by the Plant-Microbe and Soil interaction grant provided to RR and RVS by the CSIR, Government of India.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Alok Pandey
    • 1
  • Suvendra Kumar Ray
    • 1
    • 2
  • Ramesh V. Sonti
    • 1
  • R. Rajeshwari
    • 1
  1. 1.Centre for Cellular and Molecular BiologyCouncil of Scientific and Industrial ResearchHyderabadIndia
  2. 2.Department of Molecular Biology and BiotechnologyTezpur UniversityTezpurIndia

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