Current Microbiology

, Volume 67, Issue 5, pp 590–595 | Cite as

Serological Cross-Reaction Between O-Antigens of Shigella dysenteriae Type 4 and an Environmental Escherichia albertii Isolate

  • Mohammed Ziaur Rahman
  • Selina Akter
  • Nafisa Azmuda
  • Munawar Sultana
  • François-Xavier Weill
  • Sirajul Islam Khan
  • Patrick A. D. Grimont
  • Nils-Kåre Birkeland
Article

Abstract

An environmental freshwater bacterial isolate, DM104, appearing as Shigella-like colonies on selective agar plates was found to show strong and specific serological cross-reactivity with Shigella dysenteriae type 4. Biochemical identification according to the analytical profile index, molecular serotyping by restriction of the amplified O-antigen gene cluster (rfb-RFLP), together with phylogenetic analysis of the 16S rRNA gene and multi-locus sequence analysis, identified the isolate as Escherichia albertii. rfb-RFLP of DM104, revealed a profile different from that of S. dysenteriae type 4. However, western blot analysis of extracted lipopolysaccharides demonstrated strong cross-reactivity with S. dysenteriae type 4 using specific monovalent antisera and a lipopolysaccharide gel banding profile similar to that of S. dysenteriae type 4. The observed O-antigen cross-reaction between an E. albertii isolate and S. dysenteriae extends our knowledge of the extent of O-antigen cross-reaction within the Escherichia/Shigella group of organisms, and offers the possibility of using DM104 and similar cross-reacting strains as shigellosis vaccine candidates.

Supplementary material

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Supplementary material 1 (DOC 364 kb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Mohammed Ziaur Rahman
    • 1
    • 2
    • 3
    • 4
  • Selina Akter
    • 1
    • 6
  • Nafisa Azmuda
    • 1
    • 5
  • Munawar Sultana
    • 1
  • François-Xavier Weill
    • 4
  • Sirajul Islam Khan
    • 1
  • Patrick A. D. Grimont
    • 4
  • Nils-Kåre Birkeland
    • 2
  1. 1.Department of MicrobiologyUniversity of DhakaDhakaBangladesh
  2. 2.Department of BiologyUniversity of BergenBergenNorway
  3. 3.Centre for Health and Population Researchicddr,bDhakaBangladesh
  4. 4.Institute PasteurUnite Biodiversité des Bactéries Pathogènes EmergentesParisFrance
  5. 5.Department of MicrobiologyJahangirnagar UniversityDhakaBangladesh
  6. 6.Department of MicrobiologyJessore Science and Technology UniversityJessoreBangladesh

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