Current Microbiology

, Volume 67, Issue 5, pp 537–542 | Cite as

Pyrosequencing Analysis of Oral Microbiota in Children with Severe Early Childhood Dental Caries

  • Wen Jiang
  • Jie Zhang
  • Hui ChenEmail author


Severe early childhood caries are a prevalent public health problem among preschool children throughout the world. However, little is known about the microbiota found in association with severe early childhood caries. Our study aimed to explore the bacterial microbiota of dental plaques to study the etiology of severe early childhood caries through pyrosequencing analysis based on 16S rRNA gene V1–V3 hypervariable regions. Forty participants were enrolled in the study, and we obtained twenty samples of supragingival plaque from caries-free subjects and twenty samples from subjects with severe early childhood caries. A total of 175,918 reads met the quality control standards, and the bacteria found belonged to fourteen phyla and sixty-three genera. Our results show the overall structure and microbial composition of oral bacterial communities, and they suggest that these bacteria may present a core microbiome in the dental plaque microbiota. Three genera, Streptococcus, Granulicatella, and Actinomyces, were increased significantly in children with severe dental cavities. These data may facilitate improvements in the prevention and treatment of severe early childhood caries.


Dental Caries Dental Plaque Early Childhood Caries Actinomyces Plaque Sample 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We acknowledge Dr. Jinhua FANG and colleagues from Affiliated Hospital of Stomatology, Zhejiang University for their clinical assistances. The study was founded by grants 2011 China State key clinical department grants.

Supplementary material

284_2013_393_MOESM1_ESM.docx (24 kb)
Supplementary material 1 (DOCX 24 kb)


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of Conservative Dentistry and Periodontics, Affiliated Hospital of Stomatology, College of MedicineZhejiang UniversityHangzhouChina

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