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Current Microbiology

, Volume 67, Issue 1, pp 51–60 | Cite as

Phylogenetic Analysis of Burkholderia Species by Multilocus Sequence Analysis

  • Paulina Estrada-de los Santos
  • Pablo Vinuesa
  • Lourdes Martínez-Aguilar
  • Ann M. Hirsch
  • Jesús Caballero-Mellado
Article

Abstract

Burkholderia comprises more than 60 species of environmental, clinical, and agro-biotechnological relevance. Previous phylogenetic analyses of 16S rRNA, recA, gyrB, rpoB, and acdS gene sequences as well as genome sequence comparisons of different Burkholderia species have revealed two major species clusters. In this study, we undertook a multilocus sequence analysis of 77 type and reference strains of Burkholderia using atpD, gltB, lepA, and recA genes in combination with the 16S rRNA gene sequence and employed maximum likelihood and neighbor-joining criteria to test this further. The phylogenetic analysis revealed, with high supporting values, distinct lineages within the genus Burkholderia. The two large groups were named A and B, whereas the B. rhizoxinica/B. endofungorum, and B. andropogonis groups consisted of two and one species, respectively. The group A encompasses several plant-associated and saprophytic bacterial species. The group B comprises the B. cepacia complex (opportunistic human pathogens), the B. pseudomallei subgroup, which includes both human and animal pathogens, and an assemblage of plant pathogenic species. The distinct lineages present in Burkholderia suggest that each group might represent a different genus. However, it will be necessary to analyze the full set of Burkholderia species and explore whether enough phenotypic features exist among the different clusters to propose that these groups should be considered separate genera.

Keywords

Burkholderia nifH Gene Acetylene Reduction Activity Melioidosis Burkholderia Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

MLSA

Multilocus sequence analysis

BCC

Burkholderia cepacia complex

ML

Maximum likelihood

NJ

Neighbor-joining

Notes

Acknowledgments

This work is dedicated to the memory of Dr. Jesus Caballero Mellado (1953–2010), for his many years of fruitful work, support, generosity, and friendship. We are grateful to Jorge Eduardo Buendia Buendia, Isaac Fernando Lopez Moyado, Mariana del Rosario Ruiz Velasco Leyva, Jorge Arturo Zepeda Martinez, and Marie Lisandra Zepeda Mendoza for technical support during training as students from the Undergraduate Program on Genomic Sciences (Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México). We also thank Annette A. Angus (University of California, Los Angeles) for sharing her unpublished data with us. The housekeeping gene sequences were provided from the ongoing sequencing project of B. unamae MTl-641T, B. tuberum STM678T, B. silvatlantica SRMrh-20T, and B. silvatlantica PVA5 from a project, funded in part by the U.S. National Science Foundation (Grant IOB-0537497) to George Weinstock (Washington University, St. Louis, MO) and AMH.

Supplementary material

284_2013_330_MOESM1_ESM.pdf (501 kb)
Supplementary material 1 (PDF 500 kb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Paulina Estrada-de los Santos
    • 1
    • 2
  • Pablo Vinuesa
    • 1
  • Lourdes Martínez-Aguilar
    • 1
  • Ann M. Hirsch
    • 3
  • Jesús Caballero-Mellado
    • 1
  1. 1.Centro de Ciencias GenómicasUniversidad Nacional Autónoma de MéxicoCuernavacaMexico
  2. 2.Departamento de MicrobiologíaEscuela Nacional de Ciencias Biológicas, I.P.N.MexicoMexico
  3. 3.Department of Molecular, Cell and Developmental Biology, Molecular Biology InstituteUniversity of California, Los AngelesLos AngelesUSA

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