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Current Microbiology

, Volume 66, Issue 6, pp 606–612 | Cite as

Euryhalocaulis caribicus gen. nov., sp. nov., a New Members of the Family Hyphomonadaceae Isolated from the Caribbean Sea

  • Wenchao Deng
  • Yao Zhang
  • Xiabing Xie
  • Zihao Zhao
  • Yingnan Fu
Article

Abstract

A new aerobic, Gram-negative, chemo-organotrophic, euryhaline bacterium, designated strain JL2009T, was isolated from surface water of the Caribbean Sea. The strain formed flaxen colonies on marine ager 2216 (MA; Difco) medium. Cells were dimorphic, with stalks or a polar flagellum, and reproduction occurred by means of binary fission. Growth occurred at 15–45 °C (optimum at 35 °C), 0–15 % (w/v) NaCl (optimum at 1–10 %) and pH 5–9 (optimum at pH 7–8). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct evolutionary lineage within the family Hyphomonadaceae. Strain JL2009T was most closely related to Maricaulis parjimensis MCS 25T (92.2 % DNA sequence similarity), Woodsholea maritime CM2243T (90.9 %), and Oceanicaulis alexandrii C116-18T (90.9 %). The major respiratory quinone was Q-10. The dominant cellular fatty acids were summed feature 8 (C18:1 ω7c), C18:0 and 11-methyl C18:1 ω7c. The polar lipid pattern indicated the presence of phospholipid, phosphatidyl glycerol and glycolipids. The G + C content of the genomic DNA was 70.5 mol%. On the basis of the chemotaxonomic and phenotypic characteristics and the phylogenetic evidence, strain JL2009T represents a novel genus and species in the family Hyphomonadaceae, for which the name Euryhalocaulis caribicus gen. nov., sp. nov. is proposed. The type strain of Euryhalocaulis caribicus is JL2009T (=CGMCC 1.12036T = JCM 18163T).

Keywords

Euryhalocaulis caribicus Phylogeny High G + C content Euryhalinous 

Notes

Acknowledgments

We thank Dr. B.J. Tindall and the DSMZ Identification Service, Braunschweig, Germany, for determining chemotaxonomic properties of polar lipids. We also thank Drs. C.-P. Yu and A.Y. Hu, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China, for examining DNA G + C contents. This work was supported by the NSFC projects 41176095, 91028001, 41121091 and 41023007, the MOST 973 program 2013CB955700, the NSFF project 2012J01182, and the OPWSR project 201105021.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Wenchao Deng
    • 1
  • Yao Zhang
    • 1
  • Xiabing Xie
    • 1
  • Zihao Zhao
    • 1
  • Yingnan Fu
    • 1
  1. 1.State Key Laboratory of Marine Environmental ScienceXiamen UniversityXiamenPeople’s Republic of China

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