Euryhalocaulis caribicus gen. nov., sp. nov., a New Members of the Family Hyphomonadaceae Isolated from the Caribbean Sea
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A new aerobic, Gram-negative, chemo-organotrophic, euryhaline bacterium, designated strain JL2009T, was isolated from surface water of the Caribbean Sea. The strain formed flaxen colonies on marine ager 2216 (MA; Difco) medium. Cells were dimorphic, with stalks or a polar flagellum, and reproduction occurred by means of binary fission. Growth occurred at 15–45 °C (optimum at 35 °C), 0–15 % (w/v) NaCl (optimum at 1–10 %) and pH 5–9 (optimum at pH 7–8). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct evolutionary lineage within the family Hyphomonadaceae. Strain JL2009T was most closely related to Maricaulis parjimensis MCS 25T (92.2 % DNA sequence similarity), Woodsholea maritime CM2243T (90.9 %), and Oceanicaulis alexandrii C116-18T (90.9 %). The major respiratory quinone was Q-10. The dominant cellular fatty acids were summed feature 8 (C18:1 ω7c), C18:0 and 11-methyl C18:1 ω7c. The polar lipid pattern indicated the presence of phospholipid, phosphatidyl glycerol and glycolipids. The G + C content of the genomic DNA was 70.5 mol%. On the basis of the chemotaxonomic and phenotypic characteristics and the phylogenetic evidence, strain JL2009T represents a novel genus and species in the family Hyphomonadaceae, for which the name Euryhalocaulis caribicus gen. nov., sp. nov. is proposed. The type strain of Euryhalocaulis caribicus is JL2009T (=CGMCC 1.12036T = JCM 18163T).
KeywordsEuryhalocaulis caribicus Phylogeny High G + C content Euryhalinous
We thank Dr. B.J. Tindall and the DSMZ Identification Service, Braunschweig, Germany, for determining chemotaxonomic properties of polar lipids. We also thank Drs. C.-P. Yu and A.Y. Hu, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China, for examining DNA G + C contents. This work was supported by the NSFC projects 41176095, 91028001, 41121091 and 41023007, the MOST 973 program 2013CB955700, the NSFF project 2012J01182, and the OPWSR project 201105021.
- 1.Abraham WR, Bennasar A, Vancanneyt M, Snauwaert C, Swings J, Smit J, Moore ERB (2002) Phylogeny of Maricaulis Abraham et al. 1999 and proposal of Maricaulis virginensis sp. nov., M. parjimensis sp. nov., M. washingtonensis sp. nov. and M. salignorans sp. nov. Int J Syst Evol Microbiol 52:2191–2201PubMedCrossRefGoogle Scholar
- 4.Baur A, Kirby W, Sherris J, Turch M (1966) Antibiotic susceptibility testing by standardized single disc method. Am J Clin Pathol 45:493–496Google Scholar
- 6.Dong X, Cai M (2001) Determinative manual for routine bacteriology. Scientific Press, Beijing English translationGoogle Scholar
- 9.Gerhardt P, Murray R, Wood WA, Krieg NR (1994) Methods for general and molecular bacteriology. American Society for Microbiology, WashingtonGoogle Scholar
- 10.Hiraishi A, Ueda Y, Ishihara J (1998) Quinone profiling of bacterial communities in natural and synthetic sewage activated sludge for enhanced phosphate removal. Appl Evoiron Microbiol 64:992–998Google Scholar
- 11.Johnson J (1994) Similarity analysis of DNAs. In: Gerhardt P, Murray ERG, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, pp 655–681Google Scholar
- 18.Lee SH, Shim JK, Kim JM, Choi HK, Jeon CO (2011) Henriciella litoralis sp. nov., isolated from a tidal flat, transfer of Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. and emended description of the genus Henriciella. Int J Syst Evol Microbiol 61:722–727PubMedCrossRefGoogle Scholar
- 22.Poindexter JS (1964) Biological properties and classification of the Caulobacter group. Bacteriological Rev 28:231Google Scholar
- 25.Smibert R (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, pp 607–654Google Scholar
- 26.Strömpl C, Hold GL, Lünsdorf H, Graham J, Gallacher S, Abraham WR, Moore ERB, Timmis KN (2003) Oceanicaulis alexandrii gen. nov., sp. nov., a novel stalked bacterium isolated from a culture of the dinoflagellate Alexandrium tamarense (Lebour) Balech. Int J Syst Evol Microbiol 53:1901–1906PubMedCrossRefGoogle Scholar
- 29.Tindall B, Sikorski J, Smibert R, Krieg N (2007) Phenotypic characterization and the principles of comparative systematics. In: Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM, Snyder LR (eds) Methods for general and molecular microbiology 3rd edn. ASM Press, Washington, pp 330–393Google Scholar