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Identification of Bombyx mori Bidensovirus VD1-ORF4 Reveals a Novel Protein Associated with Viral Structural Component

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Abstract

Bombyx mori bidensovirus (BmBDV) VD1-ORF4 (open reading frame 4, ORF4) consists of 3,318 nucleotides, which codes for a predicted 1,105-amino acid protein containing a conserved DNA polymerase motif. However, its functions in viral propagation remain unknown. In the current study, the transcription of VD1-ORF4 was examined from 6 to 96 h postinfection (p.i.) by RT-PCR, 5′-RACE revealed the transcription initiation site of BmBDV ORF4 to be −16 nucleotides upstream from the start codon, and 3′-RACE revealed the transcription termination site of VD1-ORF4 to be +7 nucleotides downstream from termination codon. Three different proteins were examined in the extracts of BmBDV-infected silkworms midguts by Western blot using raised antibodies against VD1-ORF4 deduced amino acid, and a specific protein band about 53 kDa was further detected in purified virions using the same antibodies. Taken together, BmBDV VD1-ORF4 codes for three or more proteins during the viral life cycle, one of which is a 53 kDa protein and confirmed to be a component of BmBDV virion.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31000080, 31270192 and 31272507), China Postdoctoral Science Foundation funded project (No. 2011M500855), National basic Research Program of China under Grant No. 2012CB114604, and starting fund projects for senior professionals (09JDG057).

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Authors and Affiliations

  1. Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang, 212013, China

    Guohui Li, Zhaoyang Hu, Xuli Guo, Guangtian Li, Qi Tang, Peng Wang, Keping Chen & Qin Yao

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  1. Guohui Li
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  2. Zhaoyang Hu
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  3. Xuli Guo
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  4. Guangtian Li
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  7. Keping Chen
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  8. Qin Yao
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Correspondence to Qin Yao.

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Li, G., Hu, Z., Guo, X. et al. Identification of Bombyx mori Bidensovirus VD1-ORF4 Reveals a Novel Protein Associated with Viral Structural Component. Curr Microbiol 66, 527–534 (2013). https://doi.org/10.1007/s00284-013-0306-9

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  • DOI: https://doi.org/10.1007/s00284-013-0306-9

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