Paenibacillus polymyxa Rhizobacteria and Their Synthesized Exoglycans in Interaction with Wheat Roots: Colonization and Root Hair Deformation
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We examined the ability of several Paenibacillus polymyxa strains to colonize wheat roots and the ability of P. polymyxa exoglycans to induce root hair deformation. For the first time, exopolysaccharides isolated from P. polymyxa were found to produce, with different intensities, various morphological changes in the root hairs of wheat seedlings, which are some of the earliest responses of plants to bacteria in the surrounding milieu. P. polymyxa 1465, giving the highest exopolysaccharide yield and the highest viscosity of aqueous exopolysaccharide solutions, was best able to colonize wheat seedling roots, and its exopolysaccharide proved to be the best in producing root hair deformation. It is suggested that P. polymyxa exoglycans have an active role in the establishment of plant–microbe associations.
KeywordsRoot Hair Wheat Seedling Azospirillum Wheat Root Paenibacillus Polymyxa
Our thanks go to Mr. Dmitry N. Tychinin (this institute) for translating the original manuscript into English.
- 7.Chenu C (1995) Extracellular polysaccharides: an interface between microorganisms and soil constituents. In: Huang PM, Berthelin J, Bollag JM et al (eds) Environmental impact of soil component interactions. CRC Lewis Publishers, Boca Raton, pp 217–233Google Scholar
- 8.Dische Z (1962) Color reactions of hexuronic acids. Methods Carbohydr Chem 1:497–501Google Scholar
- 11.Fedonenko YuP, Yegorenkova IV, Konnova SA et al (2001) Involvement of the lipopolysaccharides of Azospirilla in the interaction with wheat seedling roots. Microbiology (Moscow, Russ Fed) 70:329–334Google Scholar
- 13.Haggag WM (2007) Colonization of exopolysaccharide-producing Paenibacillus polymyxa on peanut roots for enhancing resistance against crown rot disease. Afr J Biotechnol 6:1568–1577Google Scholar
- 14.Haggag WM (2010) The role of biofilm exopolysaccharides on biocontrol of plant disease. In: Elnashar M (ed) Biopolymers, vol. 14. pp 271–284Google Scholar
- 18.Karpunina LV (2002) Rol’ agglyutiniruyushchikh belkov azotfiksiruyushchikh batsill i rizobii v zhiznedeyatel’nosti bakterii pri vzaimodeistvii s rasteniyami (Role of agglutinative proteins of nitrogen-fixing bacilli and rhizobia in the vital functions of the bacteria during their interaction with plants). Dr. Sci. (Biol.) Dissertation, Saratov (in Russian)Google Scholar
- 19.Konnova SA, Skvortsov IM, Makarov OE et al (1995) Polysaccharide complexes secreted by Azospirillum brasilense and their possible role in the interaction of bacteria with wheat roots. Mikrobiologiya 64:762–768 (in Russian)Google Scholar
- 21.Michiels KW, Croes CL, Vanderleyden J (1991) Two different modes of attachment of Azospirillum brasilense Sp7 to wheat roots. J Gen Microbiol 137:2241–2246Google Scholar
- 25.Scopes RK (1982) Protein purification: principles and practice. Springer, New YorkGoogle Scholar
- 26.Slonecker JH (1972) Gas–liquid chromatography of alditol acetates. Methods Carbohydr Chem 6:20–24Google Scholar
- 32.York GM, González JE, Walker GC (1996) Exopolysaccharides and their role in nodule invasion. In: Stacey G, Mullin B, Gresshoff PM (eds) Biology of molecular plant–microbe interactions. International Society for Molecular Plant–Microbe Interactions, St. Paul, Minn., USA, pp 325–330Google Scholar