Abstract
Suspected colonies of methicillin-resistant Staphylococcus aureus (MRSA) on chromogenic, MRSASelect (BioRad) medium were confirmed using routine microbiological methods, and a multiplex real-time PCR (n = 108). Although the specificity of MRSASelect assessed at 24 h of incubation was much higher than that of 48 h (91.4 vs. 60 %), extending the incubation time to 48 h, along with PCR confirmation, increased the total number of true positive samples by 27.8 %. These results provide a cost effective method for sensitive and specific detection of MRSA.
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Hasan, M.R., Brunstein, J.D., Al-Rawahi, G. et al. Optimal Use of MRSASelect and PCR to Maximize Sensitivity and Specificity of MRSA Detection. Curr Microbiol 66, 61–63 (2013). https://doi.org/10.1007/s00284-012-0241-1
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DOI: https://doi.org/10.1007/s00284-012-0241-1