Current Microbiology

, Volume 65, Issue 2, pp 195–201 | Cite as

Biochemical and Microbial Analysis of Ovine Rumen Fluid Incubated with 1,3,5-Trinitro-1,3,5-triazacyclohexane (RDX)

Article

Abstract

In this study, the rumen was assessed for its potential to detoxify RDX using molecular microbial ecology as well as analytical chemistry techniques. Results indicated significant loss (P < 0.05) of RDX in <8-h post incubation, and qualitative LC-MS/MS analysis showed evidence for the formation of 1-NO-RDX (M–O + HCOO) and methylenedinitramine metabolites. A total of 1106 16S rRNA-V3 clones were sequenced, and most sequences associated with either the phyla Bacteroidetes or Firmicutes. A LibCompare analysis for the RDX treatment showed an enrichment (P < 0.01) of the genus Prevotella. From these results, it can be concluded that the rumen is capable of detoxifying RDX, and the members of the genus Prevotella are linked to this detoxification.

Notes

Acknowledgments

The research was supported by a jointly funded grant by the Oregon Agricultural Experiment Station project ORE00871 and by the U.S. Department of Agriculture under project number 6227-21310-007-00D agreement nos. 58-6227-8-044 and 58-1265-6-076. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. The authors would like to thank Ms. Karen Walker for her help with HPLC, Lia Murty during the LC-MS/MS analysis, and Ms. Zelda Zimmerman for editorial assistance.

Supplementary material

284_2012_144_MOESM1_ESM.doc (44 kb)
Supplementary material 1 (DOC 43 kb)

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Environmental and Molecular ToxicologyOregon State UniversityCorvallisUSA
  2. 2.Department of Veterinary MedicineOregon State UniversityCorvallisUSA

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