Effect of the Volume-to-Surface Ratio of Cultures on Escherichia coli Growth: An Experimental and Theoretical Analysis
- First Online:
- 266 Downloads
The growth dynamics of bacterial populations are usually represented by the classical S-shaped profiles composed of lag, exponential and stationary growth phases. Although exceptions to this classical behavior occur, they are normally produced under non-standard conditions such as supply of two carbohydrates as sole carbon source. However, we here report variations in the classic S-shaped growth profiles of Escherichia coli under standard culturing conditions; explicitly, we found growth during transition to the stationary phase wherein the bacterial growth rate inversely depended on the volume-to-surface ratio of cultures (V/S); the reasons for this behavior were experimentally explored. To complement our experimental analysis, a theoretical model that rationalizes the bacterial response was developed; simulations based on the developed model essentially reproduced experimental growth curves. We consequently conclude that the effect of V/S on E. coli growth reflects an interplay between auto-catalytic bacterial growth, bacterial growth auto-inhibition, and, the relief of that inhibition.
- 4.Iguchi A, Thomson NR, Ogura Y, Saunders D, Ooka T, Henderson IR, Harris D, Asadulghani M, Kurokawa K, Dean P, Kenny B, Quail MA, Thurston S, Dougan G, Hayashi T, Parkhill J, Frankel G (2009) Complete genome sequence and comparative genome analysis of enteropathogenic Escherichia coli O127:H6 strain E2348/69. J Bacteriol 191:347–354PubMedCrossRefGoogle Scholar
- 6.Martínez H, Buhse T, Rivera M, Parmananda P, Ayala G, Sánchez J (2012) Endogenous CO2 may inhibit bacterial growth and induce virulence gene expression in enteropathogenic Escherichia coli. Microb Pathog. doi:10.1016/j.micpath.2012.04.002
- 8.Monod J (1950) La technique de culture continue: théorie et applications. Ann Inst Pasteur 79:390–410Google Scholar