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Current Microbiology

, Volume 65, Issue 1, pp 14–21 | Cite as

Molecular Characterization of Mercury Resistant Bacteria Inhabiting Polluted Water Bodies of Different Geographical Locations in India

  • Arif Tasleem Jan
  • Mudsser Azam
  • Arif Ali
  • Qazi Mohd. Rizwanul HaqEmail author
Article

Abstract

Mercury pollution is a major environmental problem that arises as a result of natural processes as well as from anthropogenic sources. In response to toxic mercury compounds, microbes have developed astonishing array of resistance systems to detoxify them. To address this challenge, this study was aimed in screening bacterial isolates for their tolerance against varied concentrations of phenylmercuric acetate. Mercury transformation by bacteria being sensitive to factors such as available carbon source, etc. that affect mer-mediated transformation, screened mercury tolerant bacteria were also studied for their tolerance to different antimicrobials and carbon sources, followed by identification using biochemical as well as 16S rRNA approach. Following identification, gene encoding organomercurial lyase catalyzing protonolytic cleavage of C–Hg bond of organic mercury was amplified using gene specific primers, cloned in pGEMT® easy vector and sequenced. Microbe-based approach using organomercurial lyase encoded by merB gene being potentially economic, provides foundation to facilitate genetic manipulation of this environmentally important enzyme to remove high concentrations of obstinate mercury using holistic, multifaceted approach for use in bioremediation through generation of transgenics or as catalyst for use in bioreactors.

Keywords

Mercury Horizontal Gene Transfer Cold Vapor Atomic Absorption Spectroscopy Mercury Resistance Yamuna River 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

One of the authors, Arif Tasleem Jan is thankful to Council of Scientific and Industrial Research (CSIR), India, for financial assistance in terms of fellowship. Authors are also thankful to Dr. Inshad (Scientist, Indian institute of Integrative Medicine, Jammu) for providing standard Pseudomonas aeruginosa ATCC 9027 strain.

Supplementary material

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Supplementary material 1 (DOC 53 kb)
284_2012_118_MOESM2_ESM.doc (148 kb)
Supplementary material 2 (DOC 148 kb)
284_2012_118_MOESM3_ESM.doc (55 kb)
Supplementary material 3 (DOC 55 kb)

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Arif Tasleem Jan
    • 1
  • Mudsser Azam
    • 1
  • Arif Ali
    • 1
  • Qazi Mohd. Rizwanul Haq
    • 1
    Email author
  1. 1.Department of BiosciencesJamia Millia IslamiaNew DelhiIndia

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