Isolation and Characterization of a Lytic Myoviridae Bacteriophage PAS-1 with Broad Infectivity in Aeromonas salmonicida
- 631 Downloads
To search for candidate control agents against Aeromonas salmonicida subsp. salmonicida infections in aquaculture, one bacteriophage (phage), designated as PAS-1, was isolated from the sediment samples of the rainbow trout (Oncorhynchus mykiss) culture farm in Korea. The PAS-1 was morphologically classified as Myoviridae and possessed approximately 48 kb of double-strand genomic DNA. The phage showed broad host ranges to other subspecies of A. salmonicida as well as A. salmonicida subsp. salmonicida including antibiotic-resistant strains. Its latent period and burst size were estimated to be approximately 40 min and 116.7 PFU/cell, respectively. Furthermore, genomic and structural proteomic analysis of PAS-1 revealed that the phage was closely related to other Myoviridae phages infecting enterobacteria or Aeromonas species. The bacteriolytic activity of phage PAS-1 was evaluated using three subspecies of A. salmonicida strain at different doses of multiplicity of infection, and the results proved to be efficient for the reduction of bacterial growth. Based on these results, PAS-1 could be considered as a novel Aeromonas phage and might have potentiality to reduce the impacts of A. salmonicida infections in aquaculture.
KeywordsAeromonas salmonicida Bacteriophage PAS-1 Myoviridae Aquaculture
This study was financially supported by Basic Science Research Program (2010-0016748) and Priority Research Centers Program (2009-0093822) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology, and by Korea Research Foundation Grant (KRF-2008-331-E00385).
Conflict of Interest
The author(s) declare that they have no competing interests.
- 3.Adams M (1959) Bacteriophages. Interscience Publishers, New YorkGoogle Scholar
- 6.Fauquet C, Mayo M, Maniloff J, Desselberger U, Ball A (2005) Virus taxonomy. VIIIth Report of the International Committee on Taxonomy of Viruses, pp 35–85Google Scholar
- 7.Han JE, Kim JH, Choresca CH, Shin SP, Jun JW, Chai JY, Han SY, Park SC (2011) First description of the qnrS-like (qnrS5) gene and analysis of quinolone resistance-determining regions in motile Aeromonas spp. from diseased fish and water. Res Microbiol. doi: 10.1016/j.resmic.2011.09.001
- 21.Popoff M (1971) Étude sur les Aeromonas salmonicida—II. Caractérisation des bactériophages actifs sur les Aeromonas salmonicida et lysotypie. Ann Rech Vét 2:33–45Google Scholar