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Current Microbiology

, 63:403 | Cite as

The Distribution and Frequency of Oral Veillonella spp. in the Tongue Biofilm of Healthy Young Adults

  • Izumi Mashima
  • Arihide Kamaguchi
  • Futoshi NakazawaEmail author
Article

Abstract

Five species of oral Veillonella, V. atypica, V. denticariosi, V. dispar, V. parvula, and V. rogosae, have been suggested to be early colonizers of dental biofilm and causes of opportunistic infections and oral malodor. However, the pathogenicity and the distribution of oral Veillonella spp. have not been clarified. Previously, oral Veillonella spp. were identified by using 16S rDNA sequence analysis. In addition, recently, Veillonella isolates from human tongue biofilm were identified by rpoB gene sequences, but these procedures are time-consuming and complex. To overcome this problem, Igarashi et al. have designed species-specific primer sets for oral Veillonella spp. by using a highly variable region in the rpoB gene. In the present study, the distribution and frequency of oral Veillonella spp. in the tongue biofilm of healthy adults in their 20s were examined by using these species-specific primer sets. Tongue biofilms of these subjects were found to be divided into two groups based on the distribution and frequency of oral Veillonella spp. In one group, V. rogosae was the predominant species; the other group consisted of mainly V. dispar and V. atypica. Multiple factors may influence these differences in distribution and frequency of oral Veillonella spp. in tongue biofilm. This is the first report also demonstrating the availability of the species-specific primer sets for PCR to determine the distribution and frequency of oral Veillonella spp. in the tongue biofilm of healthy adults in their 20s.

Keywords

Early Childhood Caries rpoB Gene Oral Malodor Human Oral Cavity Severe Early Childhood Caries 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We acknowledge Osamu UEHARA for subject recruitment and clinical measurements, and Eiji IGARASHI, Hiroshi MIYAKAWA, and Mari FUJITA for sample processing. This study was supported in part by a “High-Tech Research Center” Project grant from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Izumi Mashima
    • 1
  • Arihide Kamaguchi
    • 1
  • Futoshi Nakazawa
    • 1
    Email author
  1. 1.Department of Oral MicrobiologyHealth Sciences University of Hokkaido Graduate School of DentistryIshikari-TobetsuJapan

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